DSO may stand for:
- Distinguished Service Order - A Military Decoration of the United Kingdom and Commonwealth
Musical Groups:
- Dallas Symphony Orchestra
- Dark Star Orchestra
- Detroit Symphony Orchestra
- Deathspell Omega, a French black metal band
- Deutsches Symphonie-Orchester Berlin
- Diablo Swing Orchestra
Information and Communication Technology:
- Device Software Optimization
- Digital Signal 0
- Digital switchover (from analogue to digital television broadcasting)
- Dynamic shared object, the term for shared libraries in Executable and Linkable Format
Other:
- Darkstar One
- Dark Sun Online
- Days Sales Outstanding
- Deep sky object
- Defense Sciences Office (DARPA)
- Digital Sampling Oscilloscope
- Directorate of Special Operations, a South African law-enforcement agency
- Direct Shipping Ore, referring to iron ore that can be shipped directly to a steel furnace
- Distribution System Operator in the electricity supply industry
- disulphide oil in petroleum industry, Merox Unit (LPG)
- Division Special Operations (Germany) Division Spezielle Operationen, part of Germany's Bundeswehr
- Dubai Silicon Oasis
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