| Color | mass (g/mol) | Absorb (nm) | Emit (nm) | ε (M-1cm-1) | |
|---|---|---|---|---|---|
| DyLight 350 | violet | 874 | 353 | 432 | 15,000 |
| DyLight 405 | violet | 793 | 400 | 420 | 30,000 |
| DyLight 488 | green | 1011 | 493 | 518 | 70,000 |
| DyLight 549 | yellow | 982 | 562 | 576 | 150,000 |
| DyLight 594 | orange | 1078 | 593 | 618 | 80,000 |
| DyLight 633 | red | 1066 | 638 | 658 | 170,000 |
| DyLight 649 | red | 1008 | 654 | 673 | 250,000 |
| DyLight 680 | far-red | 950 | 692 | 712 | 140,000 |
| DyLight 750 | near-IR | 1034 | 752 | 778 | 210,000 |
| DyLight 800 | near-IR | 1050 | 777 | 794 | 270,000 |
| Reference: [1] | |||||
The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific.[2] Antibodies conjugated to DyLight Dyes are produced by Thermo Fisher Scientific as well as a number of partners, including Jackson Immunoresearch[3], Rockland Immunochemicals, Inc.[4], AbD Serotec[5],and KPL[6]. DyLight dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology.
Historically, fluorophores such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications. These dyes have limitations for use in microscopy and other applications that require exposure to an intense light source such as a laser, because they photobleach quickly (however, lifetimes can be increased at least 10 fold using oxygen scavenging). DyLight Fluors have comparable excitation and emission spectra and are claimed to be more photostable, brighter, and less pH-sensitive. The excitation and emission spectra of the DyLight Fluor series covers much of the visible spectrum and extends into the infrared region, allowing detection using most fluorescence microscopes, as well as infrared imaging systems.[1]
To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 549 and 594, and a red (633 nm) laser for DyLight 633 and 649. The DyLight 680, 750 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.
DyLight Fluors are synthesized through sulfonate addition to coumarin, xanthene (such as fluorescein and rhodamine), and cyanine dyes. Sulfonation makes DyLight dyes negatively charged and hydrophilic. DyLight Fluors are commercially available as reactive succinimidyl-esters for labeling proteins through lysine residues, and as maleimide derivatives for labeling proteins through cysteine residues.
The Alexa Fluor Dyes from Invitrogen are a similar line of fluorescent dyes that provides an alternative to the DyLight Dyes[7].
References
- ^ a b "DyLight Reactive Dyes". Pierce Protein Research Products. 2008. http://www.piercenet.com/Products/Browse.cfm?fldID=9AA97866-4822-4AFE-B36D-49F7E1D7D653. Retrieved 2008-12-09.
- ^ "Fisher Biosciences Collaborates with Dyomics to Add Fluorescent Reagents for Protein Research". Press release. BNET. 2006-01-09. http://findarticles.com/p/articles/mi_m0EIN/is_/ai_n15989483. Retrieved 2008-12-09.
- ^ "DyLight New Fluorescent Dyes". Jackson Immunoresearch. 2008. http://www.jacksonimmuno.com/technical/DyLight.asp. Retrieved 2008-12-11.
- ^ "DyLight properties". Rockland Immunochemical. 2007. http://www.rockland-inc.com/objects/catalog/product/extras/12834_600-141-098.pdf. Retrieved 2008-12-09.
- ^ "AbD Serotec, a division of MorphoSys, signed an agreement to use Thermo Scientific's DyLight fluorescent dyes". BNET Business Network. 2007. http://findarticles.com/p/articles/mi_hb6656/is_1_16/ai_n29344653?tag=content;col1. Retrieved 2008-12-11.
- ^ "Thermo Fisher Scientific and KPL Sign Technology Agreement". Thermo Fisher Scientific. 2008. http://www.piercenet.com/Objects/View.cfm?type=Page&ID=8C5C0B78-90B6-4A5E-9F19-322A56951A75. Retrieved 2008-12-11.
- ^ "Alexa Fluor Dye Comparison to Alternatives". 2008-06. http://www.invitrogen.com/etc/medialib/en/filelibrary/cell_tissue_analysis/pdfs.Par.69135.File.dat/BioProbes56-AlexaFluor488.pdf. Retrieved 2008-05-29.
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