EIF4G2: Information from Answers.com

Eukaryotic translation initiation factor 4 gamma, 2

Identifiers

EIF4G2; NAT1; AAG1; DAP5; FLJ41344; p97

External IDs

OMIM: 602325 MGI: 109207 HomoloGene: 37477

Gene ontology
Molecular function	• RNA binding • translation initiation factor activity • protein binding
Cellular component	• cytoplasm • eukaryotic translation initiation factor 4F complex
Biological process	• regulation of translational initiation • cell cycle arrest • cell death

Orthologs

Species

Human

Mouse

RefSeq

NM_001042559 (mRNA)

NM_001040131 (mRNA)

NP_001036024 (protein)

NP_001035221 (protein)

Location

Chr 11:
10.78 - 10.79 Mb

Chr 7:
110.86 - 110.87 Mb

PubMed search

[1]

[2]

Eukaryotic translation initiation factor 4 gamma 2 is a protein that in humans is encoded by the EIF4G2 gene.^[1]^[2]

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.^[3]

Interactions

EIF4G2 has been shown to interact with EIF4E^[4] and EIF3A.^[4]^[5]

References

^ Yamanaka S, Poksay KS, Arnold KS, Innerarity TL (Mar 1997). "A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme". Genes Dev 11 (3): 321–33. PMID 9030685.
^ Levy-Strumpf N, Deiss LP, Berissi H, Kimchi A (Mar 1997). "DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death". Mol Cell Biol 17 (3): 1615–25. PMID 9032289.
^ "Entrez Gene: EIF4G2 eukaryotic translation initiation factor 4 gamma, 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1982.
^ ^a ^b Gradi, A; Imataka H, Svitkin Y V, Rom E, Raught B, Morino S, Sonenberg N (Jan. 1998). "A novel functional human eukaryotic translation initiation factor 4G". Mol. Cell. Biol. (UNITED STATES) 18 (1): 334–42. ISSN 0270-7306. PMID 9418880.
^ Henis-Korenblit, S; Strumpf N L, Goldstaub D, Kimchi A (Jan. 2000). "A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation". Mol. Cell. Biol. (UNITED STATES) 20 (2): 496–506. ISSN 0270-7306. PMID 10611228.

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Shaughnessy JD, Jenkins NA, Copeland NG (1997). "cDNA cloning, expression analysis, and chromosomal localization of a gene with high homology to wheat eIF-(iso)4F and mammalian eIF-4G.". Genomics 39 (2): 192–7. doi:10.1006/geno.1996.4502. PMID 9027506.
Imataka H, Olsen HS, Sonenberg N (1997). "A new translational regulator with homology to eukaryotic translation initiation factor 4G.". Embo J. 16 (4): 817–25. doi:10.1093/emboj/16.4.817. PMID 9049310.
Imataka H, Sonenberg N (1997). "Human eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A.". Mol. Cell. Biol. 17 (12): 6940–7. PMID 9372926.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Pyronnet S, Imataka H, Gingras AC, et al. (1999). "Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E.". Embo J. 18 (1): 270–9. doi:10.1093/emboj/18.1.270. PMID 9878069.
Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK (1999). "Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G.". Mol. Cell 3 (6): 707–16. doi:10.1016/S1097-2765(01)80003-4. PMID 10394359.
Henis-Korenblit S, Strumpf NL, Goldstaub D, Kimchi A (2000). "A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation.". Mol. Cell. Biol. 20 (2): 496–506. doi:10.1128/MCB.20.2.496-506.2000. PMID 10611228.
Marcotrigiano J, Lomakin IB, Sonenberg N, et al. (2001). "A conserved HEAT domain within eIF4G directs assembly of the translation initiation machinery.". Mol. Cell 7 (1): 193–203. doi:10.1016/S1097-2765(01)00167-8. PMID 11172724.
Marissen WE, Gradi A, Sonenberg N, Lloyd RE (2001). "Cleavage of eukaryotic translation initiation factor 4GII correlates with translation inhibition during apoptosis.". Cell Death Differ. 7 (12): 1234–43. doi:10.1038/sj.cdd.4400750. PMID 11175261.
Asano K, Shalev A, Phan L, et al. (2001). "Multiple roles for the C-terminal domain of eIF5 in translation initiation complex assembly and GTPase activation.". Embo J. 20 (9): 2326–37. doi:10.1093/emboj/20.9.2326. PMID 11331597.
Pyronnet S, Dostie J, Sonenberg N (2001). "Suppression of cap-dependent translation in mitosis.". Genes Dev. 15 (16): 2083–93. doi:10.1101/gad.889201. PMID 11511540.
Henis-Korenblit S, Shani G, Sines T, et al. (2002). "The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins.". Proc. Natl. Acad. Sci. U.S.A. 99 (8): 5400–5. doi:10.1073/pnas.082102499. PMID 11943866.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Perales C, Carrasco L, Ventoso I (2003). "Cleavage of eIF4G by HIV-1 protease: effects on translation.". FEBS Lett. 533 (1-3): 89–94. doi:10.1016/S0014-5793(02)03764-X. PMID 12505164.
Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801.
Li Z, Hu CY, Mo BQ, et al. (2003). "[Effect of beta-carotene on gene expression of breast cancer cells]". Ai Zheng 22 (4): 380–4. PMID 12703993.
Qin H, Raught B, Sonenberg N, et al. (2004). "Phosphorylation screening identifies translational initiation factor 4GII as an intracellular target of Ca(2+)/calmodulin-dependent protein kinase I.". J. Biol. Chem. 278 (49): 48570–9. doi:10.1074/jbc.M308781200. PMID 14507913.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.

Protein biosynthesis: translation (prokaryotic, eukaryotic)

Ribosomal proteins

Initiation factor

Prokaryotic	PIF-1 · PIF-2 · PIF-3

Archaeal	aIF1 aIF2 aIF5 aIF6

Eukaryotic	eIF1 (AX, AY, 1B) eIF2 (α, β, γ) eIF3 (A, B, C, D, F, G, H, I, J, K, M, S6) eIF4 (A2, A3, B, E1, E2, G1, G2, G3, H) eIF5 (A, A2, B) eIF6