n.
- A red crystalline powder, C20H8O5Br4, used in textile dyeing and ink manufacturing and in coloring gasoline.
- The red sodium or potassium salt of this powder, used in biology to stain cells.
[Greek ēōs, dawn (from its color) + -IN.]
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eosin
American Heritage Dictionary:
e·o·sin |
n.
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Word Tutor:
bromeosin |
IN BRIEF: n. - A red fluorescent dye resulting from the action of bromine on fluorescein.
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Oxford Dictionary of Biochemistry:
eosin |
any of a number of similar red acidic dyes, derivatives of fluorescein, especially Eosin Y (yellowish), 2′,4′,5′,7′-tetrabromofluorescein disodium salt, Acid Red 87 (CI 45380), or Eosin B (bluish), 4′,5′-dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91 (CI 45400). They are widely used as stains in histology and hematology.
| eobiont, eobiogenesis, enzymolysis | |
| eosinophil, eosinophil cationic protein, eosinophilic |
Saunders Veterinary Dictionary:
eosin |
Any of a class of rose-colored stains or dyes, all being bromine derivatives of fluorescein; eosin Y, the sodium salt of tetrabromofluorescein, is much used in histological and laboratory procedures. See also hematoxylin and eosin stain.
Mosby's Dental Dictionary:
eosin |
(e′o-sin)
n
n
One of a pair of dyes used to color tissue samples to augment visibility under a microscope. The dyes are rose-colored, causing the cytoplasm to appear pink.
Wikipedia on Answers.com:
Eosin |
For the eosin glazing process, see Zsolnay.
Eosin is a fluorescent red dye resulting from the action of bromine on fluorescein. It can be used to stain cytoplasm, collagen and muscle fibers for examination under the microscope. Structures that stain readily with eosin are termed eosinophilic.
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Contents
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Variants
There are actually two very closely related compounds commonly referred to as eosin. Most often used is eosin Y (also known as eosin Y ws, eosin yellowish, Acid Red 87, C.I. 45380, bromoeosine, bromofluoresceic acid, D&C Red No. 22); it has a very slightly yellowish cast. The other eosin compound is eosin B (eosin bluish, Acid Red 91, C.I. 45400, Saffrosine, Eosin Scarlet, or imperial red); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition.
Eosin Y is a tetrabromo derivate of fluorescein. [1]
Eosin B is a dibromo dinitro derivate of fluorescein. [2]
Use in histology
Eosin is most often used as a counterstain to haematoxylin in H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology. Tissue stained with haematoxylin and eosin shows cytoplasm stained pink-orange and nuclei stained darkly, either blue or purple. Eosin also stains red blood cells intensely red. Eosin is an acidic dye and shows up in the basic parts of the cell, i.e. the cytoplasm. Haematoxylin however is a basic dye and shows up in the acidic part of the cell like the nucleus, where nucleic acids (DNA and RNA) are concentrated.
For staining, eosin Y is typically used in concentrations of 1 to 5 percent weight by volume, dissolved in water or ethanol.[citation needed] For prevention of mold growth in aqueous solutions, thymol is sometimes added (The science of laboratory diagnosis By John Crocker, David Burnett (relevant citation for thymol inhibits growth of fungi). A small concentration (0.5 percent) of acetic acid usually gives a deeper red stain to the tissue.
It is listed as an IARC class 3 carcinogen.
Etymology
The name Eosin comes from Eos, the Greek word for 'dawn' and the name of the Greek Goddess of the Dawn.[1]
See also
References
- Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974.
- ^ Its CAS number is [] and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C(Br)C=C1C(C4=C (C([O-])=O)C=C C=C4)=C2C=C3Br.
- ^ Its CAS number is [] and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C([N+]([O-])=O) C=C1C(C4=C(C([O-]) =O)C=CC=C4)=C2 C=C3[N+]([O-])=O.
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This entry is from Wikipedia, the leading user-contributed encyclopedia. It may not have been reviewed by professional editors (see full disclaimer)
Related topics:
 | EMB |
| H & E |
| eosinophilic leukocyte |
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Copyrights:
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 | American Heritage Dictionary. The American Heritage® Dictionary of the English Language, Fourth Edition Copyright © 2007, 2000 by Houghton Mifflin Company. Updated in 2009. Published by Houghton Mifflin Company. All rights reserved. Read more |
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| Word Tutor. Copyright © 2004-present by eSpindle Learning, a 501(c) nonprofit organization. All rights reserved. eSpindle provides personalized spelling and vocabulary tutoring online; sign up free. Read more |
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Oxford Dictionary of Biochemistry. Oxford University Press. Oxford Dictionary of Biochemistry and Molecular Biology © 1997, 2000, 2006 All rights reserved. Read more | |
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| Saunders Veterinary Dictionary. Saunders Comprehensive Veterinary Dictionary 3rd Edition. Copyright © 2007 by D.C. Blood, V.P. Studdert and C.C. Gay, Elsevier. All rights reserved. Read more |
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| Mosby's Dental Dictionary. Mosby's Dental Dictionary. Copyright © 2004 by Elsevier, Inc. All rights reserved. Read more |
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| Wikipedia on Answers.com. This article is licensed under the Creative Commons Attribution/Share-Alike License. It uses material from the Wikipedia article Eosin. Read more |
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Mentioned in
- Romanowsky stains
- hematoxylin
- EMB agar (microbiology)
- Giemsa stain
- neutrophil (histology)
- resorcin
- Zsolnay
- stain
- agar
- eosinophilic
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