Evans blue

Evans blue
IUPAC name	tetrasodium (6E,6'E)-6,6-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1E)hydrazin-2-yl-1-ylidene]bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate)
Identifiers
CAS number	314-13-6
PubChem	6321418
MeSH	Evans+blue
SMILES	[Na+].[Na+].[Na+].[Na+].[O-]S(=O)(=O)c6cc(c(N)c5c6C=C/C(=N\Nc1ccc(cc1C)c4ccc(N\N=C3/C=Cc2c(cc(c(N)c2C3=O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4)C5=O)S([O-])(=O)=O
Properties
Molecular formula	C34H24N6Na4O14S4
Molar mass	960.81 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

T-1824 or Evans Blue, also rendered as Evan's Blue, is an Azo Compound dye which has a very high affinity for serum albumin.

Because of this, it can be useful in physiology in estimating the proportion of body water contained in blood plasma.^[1]

Evans blue is also used to assess the permeability of the blood-brain barrier to macromolecules. Because serum albumin cannot cross the barrier, normally the neural tissue remains unstained.^[2] It fluoresces with excitation peaks at 470 and 540 nm and an emission peak at 680 nm. ^[3]

It was named after Herbert McLean Evans, an American chemist.

See also

• Azo compound

References

1. ^ Physiology at MCG 7/7ch02/7ch02p17
2. ^ Hawkins BT, Egleton RD (2006). "Fluorescence imaging of blood-brain barrier disruption". Journal of Neuroscience Methods 151 (2): 262–7. doi:10.1016/j.jneumeth.2005.08.006. 
3. ^ Hed J, Dahlgren C, and Rundquist, I (1983). "A Simple Fluorescence Technique to Stain the Plasma Membrane of Human Neutrophils". Histochemistry 79 (1): 105–10. doi:10.1007/BF00494347. 

External links

• el-Sayed H, Goodall S, Hainsworth R (1995). "Re-evaluation of Evans blue dye dilution method of plasma volume measurement". Clin Lab Haematol 17 (2): 189–94. PMID 8536425. 

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