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Fixed Dose Procedure (FDP), proposed in 1984, is a method to assess a substance's acute oral toxicity.[1] In comparison to older LD50 test developed in 1927, this procedure produces accurate, reproducible results using fewer animals and causing less pain and suffering.[2] This test is therefore one of the replacements for the LD50 test that is recommended by the Organisation for Economic Co-operation and Development.
FDP uses about 10 to 20 animals to find the dose producing toxicity signs but not death, and from there predicts the lethal dose. LD50 ("lethal dose 50%") uses about 60 to 80 animals to find a dose killing 50% of animals within a given time. FDP sometimes requires retesting using slightly higher or lower doses.
References
- ^ Walum E (1998). "Acute oral toxicity". Environ. Health Perspect. 106 Suppl 2: 497–503. doi:. PMID 9599698. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1533392&blobtype=pdf.
- ^ van den Heuvel MJ, Clark DG, Fielder RJ, et al. (1990). "The international validation of a fixed-dose procedure as an alternative to the classical LD50 test". Food Chem. Toxicol. 28 (7): 469–82. doi:. PMID 2210519.
See also
Up-and-down procedure
Further reading
- Stallard, N.; Whitehead, A. (1995). "Reducing animal numbers in the fixed-dose procedure". Human & Experimental Toxicology 14: 315. doi:.
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