GDI is an acronym that can stand for:
- Gasoline direct injection, a modern variant of fuel injection
- Gender-related Development Index, an indication of the standard of living in a country, developed by the United Nations
- Global Defense Initiative, a fictional military organisation in the Command & Conquer computer game series
- Gonzaga Debate Institute, a popular summer policy debate workshop for high school students
- Graphics Device Interface, a core component of Microsoft Windows operating systems
- Greens for Democracy and Independence, a faction within the Green Party of the United States initiated by Peter Camejo in 2004
- Gross domestic income, one of the National Income and Product Accounts
- Guanosine nucleotide dissociation inhibitors a protein in molecular biology which binds Rho proteins
- Los Guerreros del Infierno, a Mexican professional wrestling stable
- "God Damn Independent", college slang for someone who is not in a fraternity or sorority
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