HFS may stand for:
- Hyperfine structure
- Hexafluorosilicic acid
- WHFS (historic), 'HFS was a slang abbreviation for the radio stations with that call sign in the Washington / Baltimore area
- Hemifacial spasm, a neurologic disorder which causes involuntary spasms in one side of the face
- Hospitality Franchise Systems, (HFS) later Cendant
- Haddonfield Friends School, a Quaker school in Haddonfield, NJ
- Hatfield and Stainforth railway station, England; National Rail station code HFS
In computing
- Hardware Functionality Scan
- Hi Performance FileSystem, a file system used by the HP-UX operating system
- Hierarchical File System, a file system used by Apple Macintosh computers
- HTTP File Server
In slang
- Holy Fucking Shit
- Hidden Fun Stuff, a term used when discussing caves containing very strong enemies in the game Dwarf Fortress. Holy Fucking Shit can also be used instead, although sometimes Holy Fucking Shit also applies to a megabeast that walked into the map from the surface rather than a cave.
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