n.
A substance secreted by the buccal glands of leeches, capable of preventing coagulation by inactivating thrombin and used in medicine as an anticoagulant.
[Originally a trademark, from Latin hirūdō, leech.]
hirudin
Dictionary:
hir·u·din (hîr-ūd'n, hîr'ə-dən, -yə-) 
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n.
A substance secreted by the buccal glands of leeches, capable of preventing coagulation by inactivating thrombin and used in medicine as an anticoagulant.
| 5min Related Video: hirudin |
| Medical Dictionary: hir·u·din |
(hĭ-rūd'n, hēr'ə-dən, -yə-)
n.
n.
A substance extracted from the salivary glands of leeches and used as an anticoagulant.
| Veterinary Dictionary: hirudin |
The active principle of the buccal secretion of leeches; it prevents clotting of the blood.
| Wikipedia: Hirudin |
Hirudin is a naturally occurring peptide in the salivary glands of medicinal leeches (such as Hirudo medicinalis) that has a blood anticoagulant property. This is fundamental for the leeches’ alimentary habit of hematophagy, since it keeps the blood flowing after the initial phlebotomy performed by the worm on the host’s skin.
Contents |
Structure
In 1884, the British physiologist John Berry Haycraft discovered that the leech secreted a powerful anticoagulant, which he named hirudin, though it was not isolated until the 1950s, nor its structure fully determined until 1976. Full length, hirudin is made up of 65 amino acids. These amino acids are organised into a compact N-terminal domain containing three disulfide bonds and a C-terminal domain which is completely disordered, when the protein is un-complexed in solution.[2][3] Natural hirudin contains a mixture of various isoforms of the protein. However, recombinant techniques can be used to produce homogeneous preparations of hirudin.[4]
Biological activity
A key event in the final stages of blood coagulation is the conversion of fibrinogen into fibrin by the serine protease enzyme thrombin.[5] Thrombin is produced from prothrombin, by the action of an enzyme, prothrombinase, in the final states of coagulation. Fibrin is then cross linked by factor XIII to form a blood clot. The principal inhibitor of thrombin in normal blood circulation is antithrombin III.[4] Similar to antithrombin III, the anticoagulatant activity of hirudin is based on its ability to inhibit the pro-coagulant activity of thrombin.
Hirudin is the most potent natural inhibitor of thrombin. Unlike antithrombin III hirudin binds to and inhibits only the activity of thrombin forms with a specific activity on fibrinogen.[4] Therefore, hirudin prevents or dissolves the formation of clots and thrombi (i.e. it has a thrombolytic activity), and has therapeutic value in blood coagulation disorders, in the treatment of skin hematomas and of superficial varicose veins, either as an injectable or a topical application cream. In some aspects, hirudin has advantages over more commonly used anticoagulants and thrombolytics, such as heparin, as it does not interfere with the biological activity of other serum proteins and can also act on complexed thrombin.
It is difficult to extract large amounts of hirudin from natural sources, so a method for producing and purifying this protein using recombinant biotechnology has been developed. This has led to the development and marketing of a number of hirudin based anticoagulant pharmaceutical products such as Lepirudin (Refludan) , Hirudin derived from Hansenula (Thrombexx, Extrauma) and Desirudin (Revasc/Iprivask). Several other direct thrombin inhibitors are derived chemically from hirudin.
References
- ^ PDB 4HTC
- ^ Folkers PJM, Clore GM. et al. (1989). "Solution structure of recombinant hirudin and the Lys-47-Glu mutant: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study". Biochemistry 28 (6): 2601–2617. doi:. PMID 2567183.
- ^ Haruyama H. and Wuthrich K. (1989). "Conformation of recombinant desulfatohirudin in aqueous solution determined by nuclear magnetic resonance". Biochemistry 28 (10): 4301–4312. doi:. PMID 2765488.
- ^ a b c Rydell TJ, Tulinsky A. et al. (1991). "Refined structure of the Hirudin-Thrombin complex". J. Mol. Biol. 221 (2): 583–601. doi:. PMID 1920434.
- ^ Fenton JW 2nd, Ofosu SA et al. (1998). "Thrombin and antithrombotics". Semin Thromb Hemost 24 (2): 87–91. doi:. PMID 9579630.
See also
External links
- Hirudolab
- AgroMedic - Leech Farming,Medicinal Leeches,Malaysia Leeches(Hirudinaria Manillensis)
- Supplier of Hirudo Medicinalis TURKEY
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 | leech (annelid) |
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| Hirudinea (Leeches) (zoology) |
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 | Dictionary. The American Heritage® Dictionary of the English Language, Fourth Edition Copyright © 2007, 2000 by Houghton Mifflin Company. Updated in 2009. Published by Houghton Mifflin Company. All rights reserved. Read more |
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| Medical Dictionary. The American Heritage® Stedman's Medical Dictionary Copyright © 2002, 2001, 1995 by Houghton Mifflin Company. Read more |
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