HWI could refer to:
- Hardware Wholesalers, Inc.
- Hauptman-Woodward Medical Research Institute
- Hawk Inlet Seaplane Base, Alaska, United States; IATA airport code HWI
- The Healthy Workplace Initiative of the European Agency for Safety and Health at Work
- His Wheels International
- Horwich Parkway railway station, England; National Rail station code HWI
- High Water Interval, see Lunitidal interval
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