LFO may refer to:
- Low-frequency oscillation (or oscillator); audio technique used in the production of electronic music (or the electronic circuit used to produce it).
- London Festival Orchestra; a performing orchestra formed in the 1950s
- LFO (band); an English techno act
- LFO (group); or Lyte Funky Ones, an American pop music trio
- LFO (album); an album by the American group
- Little Fighter Online; an online game
- Light Finding Operation; mechas from the anime Eureka Seven
- Legal Framework Order, 2002
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