LIFO is an acronym that stands for "last in, first out ", also known as FILO ("first in, last out") and may refer to:
- LIFO (computing)
- FIFO and LIFO accounting
- Life Orientations, performance improvement method for individual performances, team performance and communication skills
- LIFO (magazine), a free press magazine in Athens, Greece
- LIFO (education) a layoff policy
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