Middlesex may refer to:
- Middlesex, England
- Middlesex, Belize
- Middlesex, Jamaica
- five different Middlesex County locations in North America
In the United States:
- Middlesex, New Jersey
- Middlesex, New York
- Middlesex, North Carolina
- Middlesex, Vermont
- Middlesex School, Concord, Massachusetts
- Middlesex Canal, Massachusetts
In literature:
- Middlesex (novel)
- Middlesex (poem)
See also
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