MOPS

MOPS
IUPAC name	3-morpholinopropane-1-sulfonic acid
Other names	3-(N-morpholino)propanesulfonic acid
Identifiers
CAS number	1132-61-2
PubChem	70807
SMILES	C1COCCN1CCCS(=O)(=O)O
Properties
Molecular formula	C7H15NO4S
Molar mass	209.2633
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

MOPS is the common name for the compound 3-(N-morpholino)propanesulfonic acid a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES.^[1] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound which contains a piperazine ring. With a pK_a of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.^[2]Usage above 20mM in mammalian cell culture work is not recommended.^[3]

References

1. ^ Good, N.E. et al., Biochemistry, 5, 467-477 (1966).
2. ^ Thomas, J.M. and Hodes, M.E., Analytical Biochemistry, 118, 194-196 (1981).
3. ^ Eagle, H., Science, 174, 500-503 (1971).

See also

• MES
• HEPES
• CAPSO
• HEPPS
• Tris
• Common buffer compounds used in biology

External links

• Safety information
• Sigma Aldrich Buffer Calculator - Useful tool to calculate weight, volume, or concentration from molecular weight.
• Recipe for MOPS buffer on OpenWetWare

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