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Primer extension method

Q: A nick in DNA can be detected by which method?

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Primer extension method

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EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil

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primer extension technique

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Lots and lots of genes. And DNA strands. Made into a structure.

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During DNA replication Okazaki fragments are joined together by DNA polymerase.

Remember that Okazaki fragments start with an RNA primer so RNAse H is need to remove the primer follwed by DNA plymerase to add nucleotides and finally DNA ligase to seal the single strand nick.

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