nitrophenyl

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a group that is linked to a variety of compounds to form artificial substrates for enzymes. The nitrophenol released by the enzyme action can then be measured spectrophotometrically at 400 nm (4-nitrophenol) or 420 nm (2-nitrophenol) to follow the enzyme reaction, the esterified form having a maximal absorption at a much lower wavelength. Typical substrates are 4-nitrophenylacetate (for carboxylesterase, lipase), 4-nitrophenyl-N-acetylgalactosaminide (galactosaminidase), 4-nitrophenyl-N-acetylglucosaminide (glucosaminidase), and 2-nitrophenylbutyrate (cholin-esterase).

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