nitroprusside test

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a test for free thiol groups based on the red coloration given on reaction of thiol-containing compounds with ammoniacal sodium nitroprusside, sodium nitrosopentacyanoferrate(iii), Na2[Fe(CN)5NO]. Nitroprusside also reacts with ketones and is used in tests for ketone bodies in urine. The proprietary Acetest tablets contain a mixture of glycine, sodium nitroprusside, disodium phosphate, and lactose. In the presence of glycine, acetoacetate or acetone forms a blue-purple colour. A positive reaction indicates the presence of ≥5 mg dL−1 and 10 mg dL−1 for urine and blood respectively.

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