A general anaesthetic (or anesthetic) drug is a drug that brings about a reversible loss of consciousness. These drugs are generally administered by an anaesthetist/anaesthesiologist in order to induce or maintain general anaesthesia to facilitate surgery. General anesthetics have been widely used in surgery since 1846 when William Morton for the first time administered diethyl ether to a patient and performed a painless tooth extraction. It has always been believed that general anesthetics exert their effects (analgesia, amnesia, immobility) by modulating the activity of membrane proteins in the neuronal membrane. However, the exact location and mechanism of this action are still largely unknown although a lot of research has been done in this area. There are a number of outdated and modern theories that explain anaesthetic action.
The concept of specific interactions between receptors and drugs first introduced by Paul Ehrlich [1] states that drugs act only when they are bound to their targets (receptors). However, this concept is not working well in case of general anesthetics because:
- Molecular structures of general anesthetics widely used in medicine are very simple and diverse so that there is no obvious structure–activity relationship [1] (see structures of general anesthetics widely used in medicine: 1-ethanol, 2- chloroform, 3-diethylether, 4-fluroxene, 5-halothane, 6-metheoxyflurane, 7- enflurane, 8-isoflurane, 9-desflurane, 10-sevoflurane)
- Most general anesthetics have remarkably weak affinity for their targets acting at much higher concentrations than most other drugs so that diverse side effects are inevitable [ref].
All these common features of general anesthetics made it hard for early researchers to believe that general anesthetics act in a specific manner and their action on neuronal membrane was thought to be global (through nonspecific perturbation of lipid membrane of CNS neurons) rather than through specific sites.
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Lipid solubility-anesthetic potency correlation (the Meyer-Overton correlation)
The nonspecific mechanism of general anesthetic action was first proposed by Von Bibra and Harless in 1847 [2]. They suggested that general anaesthetics may act by dissolving in the fatty fraction of brain cells and removing fatty constituents from them, thus changing activity of brain cells and inducing anaesthesia. In 1899 H. H. Meyer published the first experimental evidence of the fact that anaesthetic potency is related to lipid solubility in his article entitled "Zur Theorie der Alkoholnarkose" [3] [4] [5]. Two years later a similar theory was published independently by Overton [6].
Meyer compared the potency of many agents, defined as the reciprocal of the molar concentration required to induce anaesthesia in tadpoles, with their olive oil/water partition coefficient. He found a nearly linear relationship between potency and the partition coefficient for many types of anaesthetic molecules such as alcohols, aldehydes, ketones, ethers, and esters. The anaesthetic concentration required to induce anaesthesia in 50% of a population of animals (the EC50) was independent of the means by which the anaesthetic was delivered, i.e., the gas or aqueous phase [3] [4] [5] [7].
Meyer and Overton had discovered the striking correlation between the physical properties of general anaesthetic molecules and their potency: the greater is the lipid solubility of the compound in olive oil the greater is its anaesthetic potency [7]. This correlation is true for a wide range of anesthetics with lipid solubilities ranging over 4-5 orders of magnitude if olive oil is used as the oil phase. However, this correlation can be improved considerably in terms of both the quality of the correlation and the increased range of anesthetics if bulk octanol [8] or a fully hydrated fluid lipid bilayer [9] [10] [11] [12] is used as the “oil” phase. It was noted also that volatile anesthetics are additive in their effects (a mixture of a half dose of two different volatile anesthetics gave the same anesthetic effect as a full dose of either drug alone).
Outdated lipid hypotheses of general anesthetic action
From the correlation between lipid solubility and anaesthetic potency, both Meyer and Overton had surmised a unitary mechanism of general anesthesia. They assumed that solubilization of lipophilic general anesthetic in lipid bilayer of the neuron causes its malfunction and anesthetic effect when critical concentration of anesthetic is reached. Later in 1973 Miller and Smith suggested the critical volume hypothesis also called lipid bilayer expansion hypothesis [13]. They assumed that bulky and hydrophobic anesthetic molecules accumulate inside the hydrophobic (or lipophilic) regions of neuronal lipid membrane causing its distortion and expansion (thickening) due to volume displacement. Accumulation of critical amounts of anesthetic causes membrane thickening sufficient to reversibly alter function of membrane ion channels thus providing anesthetic effect. Actual chemical structure of the anesthetic agent per se is not important, but its molecular volume plays the major role: the more space within membrane is occupied by anesthetic - the greater is the anesthetic effect. Based on this theory, in 1954 Mullins suggested that the Meyer-Overton correlation with potency can be improved if molecular volumes of anesthetic molecules are taken into account [14]. This theory existed for over 60 years and was supported by experimental fact that increases in atmospheric pressure reverse anesthetic effect (pressure reversal effect).
Then other theories of anaesthetic action emerged mostly ‘physicochemical’ theories that took into account the diverse chemical nature of general anaesthetics and suggested that anesthetic effect is exerted through some perturbation of the lipid bilayer. Several types of bilayer perturbations were proposed to cause anesthetic effect (reviews [15] [16] [17]. ):
- changes in phase separation
- changes in bilayer thickness
- changes in order parameters
- changes in curvature elasticity
According to the lateral phase separation theory [18] anesthetics exert their action by fluidizing nerve membranes to a point when phase separations in the critical lipid regions disappear. This anesthetic-induced fluidization makes membranes less able to facilitate the conformational changes in proteins that may be the basis for such membrane events as ion gating, synaptic transmitter release, and transmitter binding to receptors.
All these outdated lipid theories generally suffer from four weaknesses [1] (full description see in sections below):
- Stereoisomers of an anesthetic drug have very different anesthetic potency whereas their oil/gas partition coefficients are similar
- Certain highly lipid soluble drugs expected to act as anesthetics, exert convulsive effect instead of anesthetic effect and therefore such drugs were termed nonimmobilizers.
- General anesthetic-induced changes in membrane density and fluidity are so small that relatively small increases in temperature can mimic these effects on membrane fluidity and density without causing anesthesia.
- Cutoff effect - addition of methylene groups to a homologous series of long chain alcohols, or alkanes, increases their lipid solubility and should thereby produce a corresponding increase in anaesthetic potency, but it was observed only to a certain "cutoff" length of the chain.
In conclusion it is important to point out that the correlation between lipid solubility and potency of general anaesthetics is a necessary but not sufficient condition for inferring a lipid target site. General anaesthetics could equally well be binding to hydrophobic target sites on proteins in the brain. The main reason that more polar general anaesthetics are less potent is that they have to cross the blood-brain barrier to exert their effect on neurons in the brain.
Objections to the outdated lipid hypotheses
1. Stereoisomers of an anesthetic drug
Stereoisomers that represent mirror images of each other are termed enantiomers or optical isomers (for example, the isomers of R-(+)- and S-(-)-etomidate) [1]. Physicochemical effects of enantiomers are always identical in an achiral environment (for example in the lipid bilayer). However, in vivo enantiomers of many general anaesthetics (eg. isoflurane, thiopental, etomidate) can differ greatly in their anaesthetic potency despite the similar oil/gas partition coefficients [19] [20] For example, the R-(+) isomer of etomidate is 10 times more potent anesthetic than its S-(-) isomer [1]. This means that optical isomers partition identically into lipid, but have differential effects on ion channels and synaptic transmission. This objection provides a compelling evidence that the primary target for anaesthetics is not the achiral lipid bilayer itself but rather stereoselective binding sites on membrane proteins that provide a chiral environment for specific anesthetic-protein docking interactions [1].
2. Nonimmobilizers
All general anesthetics induce immobilization (absence of movement in response to noxious stimuli) through depression of spinal cord functions, whereas their amnesic actions are exerted within the brain. According to the Meyer-Overton correlation the anesthetic potency of the drug is directly proportional to its lipid solublity, however, there are many compounds that do not satisfy this rule. These drugs are strikingly similar to potent general anesthetics and are predicted to be potent anesthetics based on their lipid solubility, but they exert only one constituent of the anesthetic action (amnesia) and do not suppress movement (i.e. do not depress spinal cord functions) as all anesthetics do [21] [22] [23] [24]. These drugs are referred to as nonimmobilizers. The existence of nonimmobilizers suggests that anesthetics induce different components of anesthetic effect (amnesia and immobility) by affecting different molecular targets and not just the one target (neuronal bilayer) as it was believed earlier [25]. Good example of immobilizers are halogenated alkanes that are very hydrophobic, but fail to suppress movement in response to noxious stimulation at appropriate concentrations.
3. General anesthetic-induced changes in membrane density and fluidity
Experimental studies have shown that general anesthetics including ethanol are potent fluidizers of natural and artificial membranes. However, changes in membrane density and fluidity in the presence of clinical concentrations of general anesthetics are so small that relatively small increases in temperature (~1°C) can mimic them without causing anesthesia [26]. The change in body temperature of approximately 1°C is within the physiological range and clearly it is not sufficient to induce loss of consciousness per se. Thus membranes are fluidized only by large quantities of anesthetics, but there are no changes in membrane fluidity when concentrations of anesthetics are small and restricted to pharmacologically relevant.
4. Cutoff effect
According to the Meyer-Overton correlation, the addition of methylene groups to a homologous series of any general anaesthetic (e.g. n-alcohols, or alkanes) increases their lipid solubility and thereby should produce a corresponding increase in anaesthetic potency. However, addition of methylene groups cannot make anesthetic increasingly potent without limit and at a certain chain length (cutoff chain length) the addition of just one methylene group causes the molecule to lose its ability to anaesthetise. For the n-alcohols the cutoff occurs at a carbon chain length of about 13 [27] and for the n-alkanes at a chain length of between 6 and 10, depending on the species [28].
If general anaesthetics disrupt ion channels by partitioning into and perturbing the lipid bilayer, then one would expect that their solubility in lipid bilayers would also display the cutoff effect. However, partitioning of alcohols into lipid bilayers does not display a cutoff for long-chain alcohols from n-decanol to n-pentadecanol. A plot of chain length vs. the logarithm of the lipid bilayer/buffer partition coefficient K is linear, with the addition of each methylene group causing a change in the Gibbs free energy of -3.63 kJ/mol.
The cutoff effect was first interpreted as evidence that anesthetics exert their effect not by acting globally on membrane lipids but rather by binding directly to hydrophobic pockets of well-defined volumes in proteins. As the acyl chain grows, the anaesthetic fills more of the hydrophobic pocket and binds with greater affinity. When the molecule is too large to be entirely accommodated by the hydrophobic pocket, the binding affinity no longer increases with increasing chain length. Thus the volume of the n-alkanol chain at the cutoff length provides an estimate of the binding site volume. This objection provided the basis for protein hypothesis of anesthetic effect (see below).
However, cutoff effect can still be explained in the frame of lipid hypothesis [29] [30]. In short-chain alkanols (A) segments of the chain are rather rigid (in terms of conformational enthropy) and very close to hydroxyl group tethered to aqueous interfacial region ("buoy"). Consequently, these segments efficiently redistribute lateral stresses from the bilayer interior toward the interface. In long-chain alkanols (B) hydrocarbon chain segments are located further from hydroxyl group and are more flexible than in short-chain alkanols. Efficiency of pressure redistribution decreases as the length of hydrocarbon chain increases until anesthetic potency is lost at some point. It was proposed that polyalkanols (C) will have anesthetic effect similar to short-chain 1-alkanols if the chain length between two neighboring hydroxyl groups is smaller than the cutoff [31]. This idea was supported by the experimental evidence because polyhydroxyalkanes 1,6,11,16-hexadecanetetraol and 2,7,12,17-octadecanetetraol exhibited significant anesthetic potency as was originally proposed [30].
Modern lipid hypothesis
Modern version of lipid hypothesis states that anesthetic effect happens if solubilization of general anesthetic in the bilayer causes redistribution of membrane lateral pressures [29] [32].
Each bilayer membrane has distinct profile of how lateral pressures are distributed within it. Most membrane proteins especially ion channels are sensitive to changes in this lateral pressure distribution profile. These lateral stresses are rather large and vary with depth within the membrane. According to modern lipid hypothesis change in membrane lateral pressure profile shifts the conformational equilibrium of certain membrane proteins known to be affected by clinical concentrations of anesthetics such as ligand-gated ion channels. This mechanism is also nonspecific because potency of the anesthetic is determined not by its actual chemical structure, but by positional and orientational distribution of its segments and bonds of within the bilayer. However, it was not obvious what is the exact molecular mechanism... Detailed mechanism of general anesthesia was suggested and investigated using lattice statistical thermodynamics [32]. It was proposed that incorporation of amphiphilic and other interfacially active solutes (e.g. general anesthetics) into the bilayer increases the lateral pressure selectively near the aqueous interfaces, which is compensated by decrease in lateral pressure toward the center of the bilayer. Calculations showed that general anesthesia likely involves inhibition of the opening of the ion channel in a postsynaptic ligand-gated membrane protein [32] by the following mechanism:
- Channel is trying to open in the response to nerve impulse thus increasing the cross-sectional area of the protein more near the aqueous interface than in the middle of the bilayer,
- Then the anesthetic-induced increase in lateral pressure near the interface shifts the protein conformational equilibrium to back to the closed state, since channel opening will require greater work against the higher pressure at interface. This is the first hypothesis that provided not just correlations of potency with structural or thermodynamic properties, but a detailed mechanistic and thermodynamic understanding of anesthesia.
Thus according to the modern lipid hypothesis anesthetics do not act directly on their membrane protein targets, but rather perturb specialized lipid matrices at the protein-lipid interface, which act as mediators. This is a new kind of transduction mechanism, different from the usual key-lock interaction of ligand and receptor, where anesthetic (ligand) affects the function of membrane proteins by binding to the specific site on the protein. Thus some membrane proteins are proposed to be sensitive to their lipid environment. Slightly different detailed molecular mechanism of how bilayer perturbation can influence the ion-channel was proposed in the same year. Oleamide (fatty acid amide of oleic acid) is an endogenous anesthetic found in vivo (in the cat’s brain) and it is known to potentiate sleep and lower temperature of the body through closing gap junction channel connexon [33]. The detailed mechanism is shown on the picture: the well ordered lipid(green)/cholesterol(yellow) ring that exists around connexon (magenta) becomes disordered on treatment with anesthetic (red triangles), promoting a closure of connexon ion channel. This decreases brain activity and induces lethargy and anesthetic effect.
Membrane protein hypothesis of general anesthetic action
In the early 1980s, Franks and Lieb [34] demonstrated that the Meyer-Overton correlation can be reproduced using a soluble protein. They found that two classes of proteins are inactivated by clinical doses of anaesthetic in the total absence of lipid. These are luciferases, which are used by bioluminescent animals and bacteria to produce light, [35] and cytochrome P450 [36] , which is a group of heme proteins that hydroxylate a diverse group of compounds, including fatty acids, steroids, and xenobiotics such as phenobarbital. Remarkably, inhibition of these proteins by general anaesthetics was directly correlated with their anaesthetic potencies. Luciferase inhibition also exhibits a long-chain alcohol cutoff, which is related to the size of the anesthetic-binding pocket [37].
These observations were important because they demonstrated that general anaesthetics may also interact with hydrophobic protein sites of certain proteins, rather than affect membrane proteins indirectly through nonspecific interactions with lipid bilayer as mediator [8] [38]. It was shown that anesthetics alter the functions of many cytoplasmic signaling proteins, including protein kinase C, [39] [40] however, the proteins considered the most likely molecular targets of anesthetics are ion channels. According to this theory general anesthetics are much more selective than in the frame of lipid hypothesis and they bind directly only to small number of targets in CNS mostly ligand(neurotransmitter)-gated ion channels in synapse and G-protein coupled receptors altering their ion flux. Particularly Cys-loop receptors [41] are plausible targets for general anesthetics that bind at the interface between the subunits. The Cys-loop receptor superfamily includes inhibitory receptors (GABA A, GABA C, glycine receptors) and excitatory receptors (acetylcholine receptor and 5-HT3 serotonin receptor). General anesthetics can inhibit the channel functions of excitatory receptors or potentiate functions of inhibitory receptors, respectively. Although protein targets for anesthetics have been partly identified the exact nature of general anesthetic-protein interactions still remains a mystery.
It was initially hypothesized that general anesthetic binds to its target ion channel by a key-lock mechanism and changes its structure dramatically from open to closed conformation or vise versa. However, there is lots of evidence accumulated against direct key-lock interaction of membrane proteins with general anesthetics [42] [43] [44] [45].
Various studies have shown that low affinity drugs including inhaled general anesthetics do not usually interact with their target proteins via specific lock-and-key binding mechanism because they do not change molecular structures of transmembrane receptors, ion channels and globular proteins. Based on these experimental facts and some computer simulations modern version of protein hypothesis was proposed [46] [47]. Proteins of four-α-helix bundle structural motif served as models of monomer of pentameric Cys-loop receptor because binding pockets of inhaled anesthetics are believed to be within transmembrane four-α-helix bundles of Cys-loop receptors [48]. Inhaled general anesthetic does not change structure of membrane channel but changes its dynamics especially dynamics in the flexible loops that connect α-helices in a bundle and are exposed to the membrane-water interface. It is a well known fact that dynamics of protein in microsecond-millisecond timescale is often coupled with functions of the protein. Thus it was logical to propose that since inhaled general anesthetics do not change protein structure they may exert their effect on proteins by modulating protein dynamics in a slow microsecond-millisecond timescale and/or by disrupting the modes of motion essential for function of this protein. Normal interactions between residues in protein regions (loops) at the water-lipid interface that play critical roles in protein functions and agonist binding may be disrupted by general anesthetic. Interactions within the same loop or between different loops may be disrupted by anesthetics and ultimately functions of Cys-loop receptors may be altered.
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