PIPES
AMG AllGame Guide:
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Pipes
- Release Date: 1983
- Genre: Puzzle
- Style: Action Puzzle
Game Description
A game of Pipes involves purchasing tubes of different sizes and using them to connect houses to water tanks. Before showing the customers your pipe know-how, you'll have to select the number of houses you wish to connect from one to five.The more houses that need to be hooked to the tank, the more cash you'll have to spend. Pipes are located at a warehouse and come in all shapes and sizes...and prices. Generic curved pieces are inexpensive but cross-shaped tubes will cost more, since they are used to branch water in multiple directions. There are also vertical and horizontal pipes available.
The amount of cash remaining is located in the upper portion of the screen along with the money spent and the length of the current pipe. In order to succeed, you'll have to hook the houses to the water tank without spending all the allotted cash.
~ Matthew House, All Game Guide
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This article is about the biochemical buffer PIPES. For other uses, see Pipe (disambiguation).
| PIPES | |
|---|---|
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1,4-Piperazinediethanesulfonic acid (IUPAC) |
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Other names
PIPES |
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| Identifiers | |
| CAS number | 5625-37-6  |
| Properties | |
| Molecular formula | C8H18N2O6S2 |
| Molar mass | 302.37 |
| Appearance | White powder |
| Melting point |
Decomposes above 300 °C |
| Boiling point |
Decomposes |
| Solubility in water | 1 g/L (100 °C) |
| Hazards | |
| MSDS | External MSDS |
| Main hazards | Irritant |
| NFPA 704 |
 0
1
0
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 (verify) (what is:  / ?)Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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| Infobox references | |
PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), and frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]
Applications
PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.
See also
References
- ^ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
- ^ Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
- ^ Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
- ^ Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095. http://www.jhc.org/cgi/content/abstract/33/2/110.
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