pulldown technique
Oxford Dictionary of Biochemistry:
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pulldown technique
a method for investigating protein-protein interactions in which a protein of interest is expressed as a fusion protein with glutathione-S-transferase (GST) or an epitope tag such as 6 × His or c-Myc. This is used to explore previously unknown interactions with other proteins in a mixture such as a cell extract. Protein complexes formed with the fusion protein are precipitated either with specific antibodies or with a suitable affinity adsorbent, and the nature of the bound proteins is identified by gel electrophoresis and mass spectrometry.
| puff, ptyalin, pteroyl | |
| pullulan, pullulanase, pulmonary surfactant |
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Oxford Dictionary of Biochemistry
Oxford University Press. Oxford Dictionary of Biochemistry and Molecular Biology © 1997, 2000, 2006 All rights reserved.
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