pulse-chase experiment
Oxford Dictionary of Biochemistry:
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pulse-chase experiment
a technique whereby cells growing in culture are exposed for a short period (pulse) to a radiolabelled molecule such as an amino acid and then transferred to a medium containing an excess of the nonlabelled substance for a longer period (chase). By removing samples at intervals the fate of intracellular components labelled during the pulse can be ascertained.
| pulse radiolysis, pulmonary surfactant, pullulanase | |
| pulsed field gel electrophoresis, pump, purine |
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Oxford Dictionary of Biochemistry
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