PUP is a three-letter acronym that may refer to:
Medicine, science & technology:
- PARC Universal Packet, one of the two earliest internetworking communications protocols
- Potentially unwanted program, software that one probably does not want to install, but is not as harmful as spyware, such as adware
- Puerperal urticaria of pregnancy, hives or a rash that can occur during the third trimester of pregnancy
Education:
- Polytechnic University of the Philippines
- Prison University Project, a charity supporting college-level education in San Quentin Prison in California
Politics:
- People's United Party, a political party of Belize
- Party of Unity and Progress, a political party of Guinea
- Party of People's Unity, a political party of Tunisia
- Party of Proletarian Unity, a political party of France
- Progressive Unionist Party, a political party of Northern Ireland
- Protestant Unionist Party, a former political party of Northern Ireland
Entertainment:
- The abbreviation used for the Puppetmaster Job Class in Square Enix's Final Fantasy XI
Sports:
- Physically Unable to Perform, a rule in the NFL which allow teams to designate players as "Physically Unable to Perform"
- English football team Portsmouth F.C.'s main chant "Play up Pompey!" is abbreviated to 'PUP'
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