ribonuclease protection assay
Oxford Dictionary of Biochemistry:
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ribonuclease protection assay
a method for measuring the abundance of a specific mRNA in which a short (~100 nucleotide) radioactively labelled antisense RNA probe is hybridized with a population of RNA molecules. Treatment with ribonucleases A and T1 digests all single-stranded RNA leaving double-stranded RNA intact. Autoradiography or phosphorimaging of acrylamide electrophoresis gels, on which the digestion products are run, can provide quantitative information about mRNA abundance.
| ribonuclease inhibitor, ribonuclease, ribofuranosyl | |
| ribonucleate, ribonucleic acid, ribonucleoprotein |
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ribonuclease protection assay
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Oxford Dictionary of Biochemistry
Oxford University Press. Oxford Dictionary of Biochemistry and Molecular Biology © 1997, 2000, 2006 All rights reserved.
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