| Sudan Black B | |
|---|---|
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| IUPAC name |
(2,2-dimethyl-1,3-dihydroperimidin-6-yl)-(4-phenylazo-1-naphthyl)diazene
|
| Other names | Sudan Black; Fat Black HB; Solvent Black 3; C.I. 26150 |
| Identifiers | |
| CAS number | 4197-25-5 |
| PubChem | 61336 |
| MeSH | Sudan+Black+B |
| SMILES |
CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)N=NC6=CC=CC=C6)C=CC=C3N1)C
|
| Properties | |
| Molecular formula | C29H24N6 |
| Molar mass | 456.54 g/mol |
| Melting point |
120-124 °C |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) | |
| Infobox references | |
Sudan Black B (C26H24N4O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and melting point 120-124 °C. It stains blue-black.
Sudan Black B is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV.
Sudan Black B can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids.
A use of Sudan Black B is in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.
In differentiating haematological disorders Sudan black will stain myeloblasts but not lymphoblasts.
External links
- Stains File entry
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