Thermus thermophilus is a gram negative eubacterium , aerobic used in a range of biotechnological applications, including as a model organism for genetic manipulation, structural genomics, and systems biology. The bacterium is extremely thermophilic, with an optimal growth temperature of about 65ºC-75ºC. Thermus thermophilus was originally isolated from a thermal vent environment in Japan by Tairo Oshima and Kazutomo Imahori.[1] The organism has also been found to be important in the degradation of organic materials in the thermogenic phase of composting.[2] T. thermophilus is classified into several strains, in which HB8 and HB27 are most common and their genome analyses were independently completed in 2004.[3]
Biotechnological applications of Thermus thermophilus enzymes
- rTth DNA polymerase is a recombinant thermostable DNA polymerase derived from Thermus thermophilus, with optimal activity at 70-80ºC, used in some PCR applications. The enzyme possesses efficient reverse transcriptase activity in the presence of manganese.
External links
References
- ^ Oshima & Imahori (1974). "Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from Japanese thermal spa.". Int J Syst Bacteriol. 24: 102–112.
- ^ Beffa, T; Blanc, M; Lyon, PF; Vogt, G; Marchiani, M; Fischer, JL; Aragno, M (1996). "Isolation of Thermus strains from hot composts (60ºC-80ºC)". Appl. Environ. Microbiol. 62: 1723-1727.
- ^ Henne A et al. (May 2004). "The genome sequence of the extreme thermophile Thermus thermophilus". Nat Biotechnol. 22 (5): 547–53. doi:10.1038/nbt956. PMID 15064768.
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