The three letter abbreviation TLR can mean
- Toll-like receptors, proteins constituting an important part of the immune system
- Tonic labyrinthine reflex, a primitive reflex found in newborn humans
- Twin-lens reflex camera, a photo camera with two lenses
- Texas Law Review
- Tulane Law Review
- TL1000R, a motorcycle with a 998cc v-twin engine manufactured by Suzuki
- The IATA airport code for Mefford Field, California, USA
- Tubal Ligation Reversal or Tubal reversal, surgery to open and reconnect the fallopian tube after a tubal ligation procedure
- Air Libya, Libya (ICAO airline code)
- The last resistance, MMORPG
- Nickname for St. Louis Cardinals manager Tony La Russa
- The local radio station TLR (Thanet Local Radio), now known as KMFM Thanet
- Two Legged Rat - A person undergoing experimental medical treatment
- Teaching and learning responsibility - in Great Britain, special payments awarded teachers who have taken on "significant responsibility that is not required of all classroom teachers"
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