UST is a three letter acronym that can refer to:
- Equatorial Guinea Workers' Union
- Hong Kong University of Science and Technology (HKUST)
- Unnikrishnan Software Technologies (Ernakulam)
- St. Augustine Airport in St. Augustine, Florida (IATA Airport Code UST)
- Union of Trade Unions of Chad (Union des Syndicats du Tchad)
- Universal System Tubeless, a tubeless bicycle tire system developed by Mavic
- University of St. Thomas (Texas) in Houston
- University of St. Thomas (Minnesota) in Saint Paul
- University of Santo Tomas in Manila, Philippines (see also UST Growling Tigers men's varsity team)
- University of Science and Technology (South Korea), a group of public universities and research institutions
- U.S. Smokeless Tobacco Company, a subsidiary of UST Inc.
- United States of Tara, an American comedy-drama television series
- Unresolved sexual tension
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