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I would doubt it, since they give you a preg test before first shot, but no bc is 100%, so take test or see doc. In my experience with depo, the first two shots gave me the worst cramps I have ever had, with spotting and bleeding. I took the shot for a year, and even though its not part of your question, I do NOT recommend depo for anyone. It really messes you up, also they have published reports that it causes bone mass loss after two years of it. My gyn said she would've stopped the shot before two years.

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Q: Are cramping and spotting signs of pregnancy while on Depo Provera?
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