Can you use a laptop DVD drive in my PC?
A 2.5 inch laptop hard drive can be used in a desktop computer with an appropriate adapter and mounting. Laptop CD / DVD drives have proprietary connectors. To use them, you would have to partially disassemble the drive, and then connect the aforementioned adapter.
Most modern PC's will play DVD movies. However, you need to make sure your PC has a DVD drive. Some older or very low-end PC's have only a CD player, which will not play DVD's. Also, some laptop and notebook PC's save size and weight by omitting to have a built-in DVD drive. In this case you can plug an external DVD drive into a USB port.
No. An ISO is simply an "image" of a DVD, and you will need a DVD burner in order to burn it. However there are software available such as PowerISO, that will create a "virtual" drive on your PC. This virtual drive can be loaded with any content, including an ISO file, tricking your PC into thinking that there is a DVD in that "virtual" drive with the said content