Define an enzyme?
an enzyme is a protein that acts as a biological catalyst
The optimum pH and optimum temperatures of an enzyme are simply levels that the enzyme work most effectively at. These levels contribute to factors such as the enzyme's protein structure. Different levels of pH and temperature can alter the integrity of the protein, this process is called denaturation. When an enzyme undergoes denaturation, its level of activity is inhibited by failure of the substrate to effectively attach itself to the active site.
There are actually three types of inhibition Namely: Competitive, non competitive and un competitive inhibitions Competitive inhibition the inhibitor binds the enzyme at the active site making it difficult for the substrate to bind. Non competitive inhibition , the inhibitor binds the enzyme at a site different from the active site which is known as the allosteric site. hence change the size and shape of the enzyme. Uncompetitive binds the complex formed
The theory of enzyme-substrate complex was established by Michaelis and Menton in 1913.According to their theory,enzyme combines with substrate to form enzyme-substrate complex.The transient complex has a lower energy of activation than that of substrate alone.This results in the acceleration of the reaction.The enzyme-substrate complex is broken down into enzyme and products of the reaction. E+S -ES - - -ES - - -E+P The different hypotheses of enzyme-substrate complex formation have been postulated.Early theory was…
An enzyme is one kind of protein that can catalyze a specific reaction whereas a regulatory enzyme is the enzyme which can regulate a series of reaction which undergo in the living organism. So we can say every enzyme is not a regulatory one but the regulatory enzymes are obviously a special kind of enzyme.
Enzyme activity is affected by other molecules, temperature, chemical environment (e.g., pH), and the concentration of substrate and enzyme. Activators are molecules that encourage enzyme activity, and inhibitors are enzymes that decrease enzyme activity. Sometimes a cofactor is necessary for the enzyme to work.
Increasing enzyme concentration increases the number of collisions between the enzyme molecules and the substrate molecules. This increases the number of successful collisions and the number of enzyme-substrate complexes. Therefore the reaction rate is increased as well and enzyme activity is promoted.