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No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.

An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.

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12y ago
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11y ago

Salmonella is not grown on mannitol salt agar as it is Gram- .

Gram+ bacteria are grown on mannitol salt agar.

Salmonella is grown on xysoline lysine deoxycholate (XLD) agar which is a differential media to differentiate salmonella from shegilla.

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12y ago

no, it doesn,t tolerate high salt

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Q: Can serratia marcescens grow in mannitol salt agar?
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How long does it take non pathogenic Serratia Marcescens D1 to grow and can you use nutrient agar as a medium?

Two days at room temperature on TSA (tryptic soy agar)


Can Klebsiella pneumoniae grow on Mannitol salt agar?

No, K. pneumoniae will not grow on Mannitol Salt Agar- gram negative organisms cannot grow on MSA, and K. pneumoniae is gram negative.


Why does gram-positive bacteria grow in mannitol agar?

Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê


Can Serratia grow on Hektoen Enteric Agar?

Yes. Serratia is a Gram negative rod from the Enterobacteriaceae family. The Hektoen Enteric agar allows the growth of Gram negative bacteria while inhibiting growth of Gram positive bacteria.


Can Streptococcus grow on MSA?

MSA stands for mannitol salt agar. Streptococcus can not grow on this type of agar. This is because it has a very high salt content, which allows only certain microorganisms to grow in it.


What makes Serratia Marcescens such a good bacteria for science students to grow in a culture and examine?

haha this is from 9th grade Killer Germs project. Im looking for it too:)


How do mannitol-salt agar tests work?

Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.


Why is serratia marcescens commonly used in science experiments?

Because; Let me specify;Serratia marcescens is a motile,short rod-shaped, Gram-negative, facultative anaerobe bacterium, classified as an opportunistic pathogen. It was discovered in 1819 by Bartolomeo Bizio in Padua, Italy. Bizio named the genus Serratia in honor of and Italian physicist named Serratia, and chose marcescens for the species name after the Latin word for decay [3]. Serratia marcescens was first thought to be harmless (non-pathogenic). Due to its ability to produce red pigmentation, it was first used in 1906 as a marker in order to trace bacterial activity or transmission [4]. It was not until later in the 1950's that the US government experimented with the Serratia marcescens and the harmful affects that the bacteria causes were revealed. A study using Serratia marcescenswas carried out to determine the possibility of biological weapons being transmitted by wind current. In the famous "Operation Sea Spray" the US Army filled balloons with Serratia marcescens and exploded them over San Fransisco. Serratia marcescenswas chosen because it was easily tracked due to its pigment production. However,there was an increase in the number of pneumonia and urinary tract infections [1] reported in the region shortly after the experiment was conducted . Although Serratia marcescens was classified as a human pathogen in the 1960s, scientist still used it as a bacterial tracer well into the 1970s [10].Optimally, Serratia marcescens grows at 37°C, but it can grow in temperatures that range from 5-40°C. They grow in pH levels that range from 5 to 9 [14].Serratia marcescens is well known for the red pigmentation it produces called prodigiosin. Prodigiosin is made up of three pyrrole rings [15] and is not produced at 37°C, but at temperatures below 30°C [20]. The red pigment production is not present in all strains but in those that it is present, it can resemble blood [10]. This and the fact that Serratia marcescens typically grows on bread and communion wafers stored in moist places, has led scientists to suggest Serratia contamination as a possible explanation for transubstantiation miracles (the conversion of bread to the body and blood of Christ). For example, the story of the Miracle of Bolsena states that, in 1263, a priest with doubts of Christ's presence in the consecrated Host presided over a mass in the Basilica of Bolsena. After speaking the words of consecration, blood began to drip from the consecrated Host onto his hands and the altar [1]. This event was depicted by Raphael on the walls of the Vatican [19].


What selective agent found in Mannitol Salt Agar?

The high salt concentration (7.5-10% w/v) makes the agar hard for all but staph spp. To grow, and staph aureus turns yellow when it ferments the mannitol.


Can enterobacter aerogenes grow on mannitol salt agar?

the bacteria dont ferment in MSA, there is no growth on the plat


Did lactobacillus grow on mannitol salt agar?

Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.


Purpose of high salt concentration in the mannitol salt agar medium?

Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.