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The diaphragm controls the amount of light going through the microscope. If you close it, less light will come through. If an object is light in color, this can help you see it better. If you open it, more light will come through. That will help with darker objects.

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Q: Explain the effect on the specimen if you open or close the diaphragm under the stage of the microscope?
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What is the effect on the specimen if you open or close the diaphragm under the stage of your microscope?

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