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Kd = (Ve - Vo)/(Vt - Vo)

Ve = elution volume

Vo = void volume

Vt = volume total

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Q: Formula for calculating distribution coefficient kd in size exclusion chromatography?
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Continue Learning about Chemistry

What steps should a student follow to separate a mixture?

That would depend on the properties of the substances in the mixture. You can separate mixtures based on differences in boiling points by distillation. If the substances have different isoelectric points, they can be separated by ion-exchange chromatography. If the substances are made up of molecules of significantly different size, then each type of molecule can be separated by size-exclusion chromatography. There are many more examples of ways that mixtures can be separated, but without more information it's impossible to say which would be best for your particular scenario.


The Pauli exclusion principle states that no two electrons in the same atom can?

The Pauli exclusion principle states that no two electrons in the same atom can?


Who made the statement that no more than two electrons in the same atom can occupy a single orbital?

The orbital that allows no more than two electrons is the s orbital. This is a spherical shaped orbital. Elements with valence electrons lying only in the s orbital are metals.


What are the different principles that govern the building of an electron configuration?

Pauli's Exclusion Pricipal.A single orbital can only hold 2 electrons.Hund's Rule.The most stable arrangement of electrons is that with the maximum number of unpaired electrons,all with the same spin direction.Aufbau Principle.Electrons fill the lowest energy subshell first before moving to the next subshell.


Can two electron have the same set of quantum number?

Pauli's exclusion principle

Related questions

What is molecular exclusion chromatography?

molecular exclusion chromatography is the exclusion or separation of protein particles based on their molecular size. Bhubanyu Basu


How can you separate liquid components in buckthorn berry?

size exclusion chromatography - http://en.wikipedia.org/wiki/Size_exclusion_chromatography


What are disadvatages of chromatograph?

chromatography has many varieties -paper chromatography, sometime complexe mixtures cant be separated, TLC plates do not have long stationary phases -gaz chromatography: the molecule should be volatile -Chiral Chromatography can be expensive - Ion Exchange or Ion Chromatography: Turbidity should be low below 10ppm -Size Exclusion Chromatography: low resolution technique which gives few peaks and requires large differences in molecular weight for resolution -Gel chromatography: the target protein frequently becomes an abundant substrate for proteases that may also be present in the mixture. Another disadvantage is low sample handling.


What is a sentence with the word exclusion?

Stay away from the exclusion zone.The exclusion made him think twice about his behaviour.


What rule or principle states that no two electrons in the same orbital can have the same spin?

This is the Pauli exclusion principle. Wolfgang Pauli was a Jewish physicist, Nobel prize laureate.


What is Gause's law of competitive exclusion?

Competitive exclusion principle.


How long does an exclusion on a driver stay in effect with auto insurance?

An exclusion will stay in effect until you ask the insurance company to reverse the exclusion.


When was Accessibility without Exclusion created?

Accessibility without Exclusion was created in 2010.


Make a sentence using the word exclusion?

I will do my best to prevent exclusion from my team


The exclusion of one species by another due to competition?

competitive exclusion principle


When did Asiatic Exclusion League happen?

Asiatic Exclusion League happened in 1907.


What was the first act that congress passed that restricted immigration into the US?

what was th first law passed to limit immigration?