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Ninhydrin works because it reacts with the amino acids left behind in a latent print.

Ninhydrin has a polar carbonyl carbon with is electron deficient. It is attacked by the nucleophilic nitrogren on an amino acid, temporarily combining the ninhydrin and amino acid molecule. The structure is rearranged until the origionally attacked carbon is protonated and leaves in the form of water. This creates a schiff base when the nitrogen is double bonded to the origionally attacked carbon. This molecule rearranges again so that the nitrogen is double bonded to the adjacent carbon of the amino acid. This last rearrangement produces carbon dioxide gas. Further rearrangement of the product produces ruheman's purple.

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15y ago
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14y ago

chemical that used to detect protein or specific to detect ammonia and amine.these reaction will formed a dark blue or purple colour

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12y ago

Ninhydrin is used to determine the presence of lysine.

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13y ago

Ninhydrin Test is a neurological test of sensation following peripheral nerve injury. Positive would be that there is nerve damage.

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10y ago

Amines (including a-amino acids) must be present in order for the ninhydrin test to work.

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Q: What functional group must be present for the ninhydrin test to work?
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It should react to this reagent. However it must be HEATED before it will react.This is due to the fact that when Ninhydrin is heated it stabilizes and the reacts with the -NH2 groups on the amino acid.


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