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Physiology is the study of the function of body structures including organs. In contrast, anatomy is the study of the structures.
The microscope that is used to see internal structures of cells in a natural state is the compound light microscope. The microscope has a lens and light that allows it to see the internal structures of a cell clearly.
Bacterial genomes are termed as chromatid in contrast to complex chromosome structures of eukaryotes
Chemical stains provide contrast with which to view dintinctly different portions of cell structures.
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.
An irrigation staining technique is used to increase the contrast of soft tissue structures on an imaging study. This is done by injecting a contrast agent into the tissue. The contrast agent is typically iodine-based. Iodine-based contrast agents can be irritating to the tissue and can cause inflammation. This can lead to increased pain and discomfort for the patient. Additionally, iodine-based contrast agents can cause allergic reactions in some patients. An irrigation staining technique can help to minimize these side effects by diluting the contrast agent and evenly distributing it throughout the tissue. This can help to improve the comfort of the patient and increase the safety of the procedure.
electron microscope.
individual cells are practically transparent, that is, light will pass directly through them and when viewed under a normal light microscope, almost nothing will have enough contrast for you to descern the different structures. Staining gives colour to the sample, increasing contrast between the cells and the surroundings, hence making it easier for you to observe them under the microscope. Usually stains do not simply give colour to the cell in general but bind to specific structures within the cell, making it easier to view specific organelles. However, staining is not required for all types of microscopy techniques. Some microscopy techniques required a completely different method of sample preparation, such as in electron microscopy.
The reason for staining specimens is to see certain organelles within a eukaryotic cell. These structures as well as the cell itself are normally translucent. The stain provides a contrast that will make the part one wants to see more visible. With prokaryotic cells, staining helps identify the type of bacteria. Staining also allows us to see parts of the cell that would normally be unidentifiable by the biologist. I am sure that you have seen pictures of cells in mitosis or meiosis where the chromosomes have been stained and are readily apparent.
Physiology is the study of the function of body structures including organs. In contrast, anatomy is the study of the structures.
The microscope that is used to see internal structures of cells in a natural state is the compound light microscope. The microscope has a lens and light that allows it to see the internal structures of a cell clearly.
Bacterial genomes are termed as chromatid in contrast to complex chromosome structures of eukaryotes
Bright field microscope : used by biology students Dark field microscope : Improves apparent resolution to see smaller structures in cells. Used to view unstained specimens. Phase contrast microscope : Makes many structures within a living cell highky visible without staining or altering the cell.
Uranyl acetate and uranyl formiate increase the contrast in electron microscopy of viruses, cells, biological samples, protein molecules, etc.
Staining adds contrast making the bacteria easier to see.
A necessary cause is a condition that, by and large, must be present for the effect to follow. A sufficient cause, in contrast, is a condition that more or less guarantees the effect in question.
Chemical stains provide contrast with which to view dintinctly different portions of cell structures.