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How could determine the concentration of ascorbic acid in an unknown sample?
Wiki User
∙ 13y ago
The concentration of ascorbic acid in an unknown sample can be obtain by conducting a few steps by using the DCPIP test. Firstly, we must obtain the volume (dm ) of the sample solution which are required to turn the DCPIP solution (blue) colour into colourless. After obtaining the volume we use an electronic balance to obtain the mass of the sample solution in gram (g). Then we use the concentration formula of [mass (g) of solute per volume (dm ) of solution] to find out the concentration. Hence. The concentration obtained was the concentration of ascorbic acid.
Wiki User
∙ 13y ago
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What is hypothesis of a acid base titration?
to determine the concentration of the unknown solution and to determine the molar concentration of acetic acid in a sample of vinegar by titrating it with a standard solution of NaOH.
Why is a titration performed?
To find the unknown concentration of a sample by using a reagent with a known concentration. ( IE; molarity )
What is external standard method for chromatographic technique and how to perform it?
In external standard method we run a standard for the substance we are looking for in our sample and than run our sample in the same conditions. we will obtain peaks for the sample and standards and can correlate the sample with the standards and can determine the concentration of the same in our sample. for more details contact vani2007
What is titrand?
There are answers elsewhere on the web. In summary, a titrand is the solution (or other substance) which one has in a conical flask or beaker into which the titrant is titrated from a burette.
What is the acid concentration of a sample of acid rain with a pH of 4.20?
69
How do you determine the concentration of ascorbic acid in unknown sample?
first measure the volume of the sample solution needed to change the blue color of the DCPIP solution into colourless. then, weigh the mass of the sample solution. finally calculate the concentration by using the formula: volume required t change the color of DCPIP solution (dm) per mass of the sample solution (g)
What is hypothesis of a acid base titration?
to determine the concentration of the unknown solution and to determine the molar concentration of acetic acid in a sample of vinegar by titrating it with a standard solution of NaOH.
Why is a titration performed?
To find the unknown concentration of a sample by using a reagent with a known concentration. ( IE; molarity )
What are the application of radial immunodiffusion?
1.used for determining the antigen concentration of unknown sample. 2.to identify the specific viral antigen from the mixed viral sample.
What are the applications of radial immuno diffusion?
1.used for determining the antigen concentration of unknown sample. 2.to identify the specific viral antigen from the mixed viral sample.
A sample of ascorbic acid contains 1.50 of carbon and 2.00 of oxygen while another sample of ascorbic acid contains 6.35 of c. How many grams of oxygen does it contain?
23
How do you make a standard curve graph?
Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis. Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples. To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis. In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar. Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
What is the purpose of wavelength scan?
Wavelength scans measure the absorbance and emission of light through a sample. Absorbance is proportional to concentration and a wavelength scan can be used to determine concentrations of a sample.
Why should the concentration of sample be low in spectrophotometer readings?
When doing reading on a spectrophotometer, the sample being studied is either a color change or a precipitated compound, depending on the wavelength that it is being read. If it is a precipitated compound and it has a very high concentration, then you run the risk of the light being used to measure the absorbance not going through. In which case you have total absorbance but it is inaccurate in helping you determine the concentration of your sample because you are unsure where the concentration limit is for that wavelength, and your sample could possibly be able to absorb more. In which case you still can't calculate the concentration of the sample.
What is an rf value used for?
The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.
Determining the concentration of a solution?
To determine the concentration of a solution, there are several options. You can evaporate the liquid off and measure what remains. You could also analyze a sample of the solution to determine the exact components.
What condition should use the standard addition method?
The standard addition would be used when determining the concentration of a sample because of matrix effect problems (matrix effect occurs when unknown sample contains many impurities).
