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How could the pour plate procedure be used for counting the number of bacteria in a sample?
Wiki User
∙ 9y ago
It is recommended that plates that should be counted should be between 30 and 300 McCance and Harrigan (1992). When more that 300 colonies you cant count them with great accuracy and represented with TNC ( too numerous to count). If colonies are less that 30 do you conclude that there was no bacteria in a product or food, if counting less than 20 colonies is unrealistic. Some standards are zero tolerant where even one colony means alot. So IDF (19991/1992) came up with a formula which takes in account the plates with/ even less than 30 colonies. The formula takes into account all sums of colonies obtained on the pour plates to come up with total cfus
Wiki User
∙ 12y ago
Wiki User
∙ 9y agoThe pour plate procedure can be used to count the number of bacteria in a sample. This is done by counting the colonies and estimating the number of bacteria total across all colonies.
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