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It is also called "miniprep method". there certain other methods but i know about the miniprep method.
It involves following basic steps:
1. source to get the DNA like some samples
2. increasing its quantity by PCR
3. isolating the DNA out of unwanted substance into the pure form
1. first the DNA containing solution is kept at the temp of 4 degree celsius so that all the enzymes get inactivated.
2. it is treated with an anionic detergent so that the DNA makes a complex with it.
3. the whole solution is treated with hot beta-merceptoethanol so that all the protein present gets denatured.
4. it is then added with phenol:chloroform in the 24:1 ratio so that we get 2 layers.....
a) upper organic layer which contains the nucleic acid i.e.DNA and RNA
b) lower aqueous layer with cell debris and other unwanted material.
5. the upper layer is decanted in another test tube
6, now RNAse is added to remove RNA
7. now NaOH is added to remove an anionic detergent.
8. now ethanol is added to precipitate out the DNA in the form of smooling structure.
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First isolated DNA using pus collected from bandages at a local hospital since white blood cells are a major component of pus they were your source of DNA?
Friderich Miescher
How long after a crime can identifiable DNA evidence be collected from sperm found on clothing?
Too broad a question to answer here. It all depends on the type(s) of evidence being collected and the conditions to which it has been exposed.
Compare the banding patters formed on each lane of the gel do you think the three DNA samples tested are the same explain how can you further verify wheter or no any of the DNA samples tested are?
It depends on the data you collected. There is NO way to figure out if the DNA samples were the same, without the actual data.
Why does addition of ice-cold ethanol lead to the precipitation of DNA?
Because it need to preserve for DNA expert to use. Just like all the living things if an animals is died in snow, snow are used to preserve them. Same as DNA
What are the benefits and the limitations to using DNA to solve crimes?
The benefits of using DNA to solve crime are many. Every human has their own DNA which means that no matter how well someone tries to hide their identity, they can never change their DNA. This translates to being able to use DNA to track down a criminal or identify a victim's body. The limitations of the process are many though as DNA can wash away before it is collected.
When DNA profiling is carried out the second DNA sample the one not collected at the crime scene is called the?
mitochondrial sample
Can DNA be collected after embalming and if so from what areas?
Teeth are a very common place to get DNA when you can't get any DNA from any other place. There is tissue inside your teeth that are very rich in DNA.
How do you get DNA in dino run?
you get 1 DNA for every 8 eggs collected/critters eaten, so rainin lizards is a good speedrun for getting lots of DNA.
Can a mouth swab sent away for DNA testing determine the age of the person it was collected from?
No, it can only be used to generate a DNA profile.
Is a DNA test right if it is taken from the people on different dates and if so can you get the case thrown out?
Because DNA is unchangeable, the date it's collected is irrelevant. It can be collected within an hour or in thrity years; it's still the same DNA. Testing methods and handling of the samples can affect the test results.
How can DNA solve crimes?
It can be used to identify the criminals easily if the criminal's hair sample or blood sample is collected and comparing it with the DNA of the suspects
Why are most hair specimens collected at crimes scenes are not good souces of DNA?
dudi
Who isolated DNA using pus collected from bandages at a local hospital?
Friedrich Miescher
Which scientist first isolated DNA using pus collected from bandages at a local hospital?
Friedrich Miescher
You first isolated DNA using pus collected from bandages at a local hospitalsince white blood cells are a major compnent of puthey were your source of DNA?
Friedrich Miescher
First isolated DNA using pus collected from bandages at a local hospital since white blood cells are a major component of pus they were your source of DNA?
Friderich Miescher
How long after a crime can identifiable DNA evidence be collected from sperm found on clothing?
Too broad a question to answer here. It all depends on the type(s) of evidence being collected and the conditions to which it has been exposed.
