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How do you make 20 mM potassium dihydrogen phosphate buffer with only dihydrogen?
Wiki User
∙ 10y ago
Without phosphate you can't.
Wiki User
∙ 10y ago
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How do you make 0.04 M phosphate buffer with pH 6?
Cfjn
How do you make 0.5 M potassium dihydrogen orthophosphate?
KH2PO4=136 weigh 68g dissolve in 1 liter of water
How do you make a sodium phosphate buffer solution of pH 6.8?
For pH = 7,0: 756 mL disodium hydrogen phosphate (Na2HPO4) solution 0,1 M (14,2 g/L) + 244 mL hydrochloric acid (HCl) solution 0,1 M
How do you make a di sodium hydrogen phosphate buffer solution of pH 3.0?
dude i cant tell you way to private you better know this all ready use your bean ok it helps you to learn
What can be mixed in solution with H2CO3 to make a buffer?
H2CO3 is not used as buffer.
What compounds make a buffer solution with a pH of 7 neutral?
For the preparation of a solution with the pH=7,00:Add 29,1 mL sodium hydroxide solution 0,1 M to 50 mL potassium dihydrogen phosphate solution 0,1 M.
How can you make 50mM potassium phosphate buffer of ph5 from dihydrogen potassium phosphate and dipotassium monohydrogen phosphate?
It is not a good proposal for making a buffer pH=5, and here it's why:Acid / base mixtures act best as buffers if their pH differs not more than 1.5 unit (up&down) from the pKa value of the acid in that acid/base mixture.So for KH2PO4(acid part) and K2HPO4 (base part) the pHbuffer should be between limits: 5.7 and 8.7 because pKa (= pKKH2PO4) = 7.2 .Otherwise the buffer does not function properly as buffer.So if you want to use this 'couple' as buffer at pH=5.0 it won't work.I would suggest to use a more acidic 'couple' eg. Acetic acid / acetate : pKacid = 4.77You should use the molar ratio of 0.54 mole 'Acetic acid' to 1.00 mole 'Acetate'
How do you make 0.04 M phosphate buffer with pH 6?
Cfjn
Is there any calculation to make phosphate buffer at pH 7?
Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html
How do you prepare phosphate buffer PH 6.8?
dissolve the 12 g of crystals of sodium phosphate in water to make 1oo ml
How do you make 0.5 M potassium dihydrogen orthophosphate?
KH2PO4=136 weigh 68g dissolve in 1 liter of water
How do you prepare phosphate buffer of pH 7.5?
See the Related Links for "Columbia.edu: Phosphate buffer automatic calculator" to the bottom for the answer.
What chemical and physical elements make up phosphate?
There are three different chemical and physical elements that make up phosphate. These elements are oxygen, potassium and sodium.
What is the dissolution method for atorvastatin tablets if you want to analyze by uv spectroscopy?
the best conditions for atorvastatin calcium 10 mg tablets is as the following dissolution media is 0.05 potassium dihydrogen phosphate buffer PH is 6.8 use paddle II 75 rpm for 45 min make standard dilution in the same media and max abs. is almost at 243 nm for higher conc of atorvastatin use sodium lauryl sulphate 0.5 %
How do you make this solution?
I don't know how to make the solution below. Low salt buffer: 10 mM phosphate buffer, 10 mM NaCl, pH 7.4. Could you tell me the method in detail?
How do you make urease solution?
you can prepare 20nM sodium phosphate buffer pH7.0. calculate mass of enzyme and dilute in volume buffer to reach concentration you want. good luck
How do you make 50 mM potassium phosphate buffer pH 7.4?
1 M Sodium Phosphate Buffer Stock Solution (1 liter) Protocol # Solution A: Dissolve 138.0 g NaH2PO4?H2O in 1 liter dH2O (pH 7.0). # Solution B: Dissolve 142.0 g Na2HPO4 in 1 liter dH2O (pH 7.0). # Mix 423 ml Solution A with 577 ml Solution B. # Autoclave and store at room temperature.
