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How do you make a 10 micromolar solution from a 100 micromolar stock solution?
Wiki User
∙ 14y ago
5 millimolar (5 thousandths of a mole per liter) is equal to 5000 micromolar ( 5000 millionths of a mole per liter). To make a 50 micromolar solution from 5 millimolar stock solution, you therefore need 5000/50 = 100 fold dilution.
Remove a 10 ml aliquot of stock and transfer to a 1000ml (1 liter) volumetric flask. Dilute with the solvent -usually water, and fill up to the graduation mark. You will now have 1 liter (1000 ml) of 50 micromolar solution.
Wiki User
∙ 10y ago
Wiki User
∙ 10y ago- Divide the molar mass of the compound with 1 000 000.
- Multiply with 200.
- Weigh this mass if it is possible; alternatively obtain this mass from a stock solution.
- Dissolve this compound in 1 L of demineralized water.
Wiki User
∙ 15y agoDissolve 2ml from stock in 8ml of diluent.
Wiki User
∙ 14y agotake 1ml from stock solution and dilute to 10ml of water to get 10 micromolar solution
Wiki User
∙ 11y agodilute it twofold. this means if you choose a 100ml volumetric, take 50ml of your stock and fill it to the mark (another 50ml) with solvent
Wiki User
∙ 11y agoAny dilution of 100,000 (= 1,000,000 / 10) will do, eg. 1 mL added up to 100 L.
Wiki User
∙ 13y agoThe easiest way would be to take a 25 ml aliquot of the 50 micromolar solution with a pipette and transfer to a 50 ml volumetric flask and then make up to the mark with distilled water.
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