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Aseptic technique correct sequence of events in transferring bacteria from a culture tube into a new media tube?
Wiki User
∙ 13y ago
1) Light a Bunsen burner and hold a transfer loop (*or needle) over the flame until it becomes red hot (hottest place is just above the first blue flame).
a. *loops are used for transfers to broth media, needles are for transfer to agar media
2) Hold both test tubes in one hand at an angle (be sure not to spill, use your non-dominate hand, and have the mouth sections of the tubes set up above your hand). In the other hand you should be holding the sterile transfer tool with your thumb and forefinger, take the tube caps off with your other three fingers. (be sure note which cap goes with which tube and not to mix them up)
3) Quickly pass the top sections of the tubes through the Bunsen flame.
4) Dip the needle or loop into the broth culture and stir around.
a. When using a loop be sure to get a drop in the middle.
5) Take out the tool without touching the sides of the tube and transfer the bacteria into the other tube media. (loops for broth media should be stirred, needles for agar media should be stabbed straight down almost to the butt of the tube and takes back straight out)
6) Take the inoculation tool out (continue holding) and sterilize the tube tops again (having been kept at a slant the entire time) and replace the correct caps.
7) Sterilize the inoculation tool in the Bunsen flame again before putting it down anywhere.
Wiki User
∙ 13y ago
Wiki User
∙ 12y agoLight up Bunsen burner,around the burner is sterile. and follow the standard aseptic transfer
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How is possible to contaminate subculturing?
By improperly handling of the bacteria, or by doing any or all steps of the aseptic technique incorrectly.
Why is aseptic technique necessary for successful cultivation of pure cultures in the laboratory?
Short answer is Koch's postulates. Long answer you can't ascribe any scientific data you may gather to a bunch of bacteria on a plate or in a culture, as you won't know which of the bacteria gave you the result you observed. So if you are trying to figure out which bacteria causes a disease, inoculating a mix of bacteria won't tell you which one causes the disease. Isolating each one using aseptic technique and then inoculating individually and finding the disease in one animal will tell you which bacteria caused to disease.
What is the difference between aseptic and antiseptic?
The adjectives are partly synonymous, but something that is antiseptic kills bacteria, while something aseptic is "surgically clean or sterile."
When you access a port catheter do you use is sterile technique or aseptic?
The terms sterile and aseptic mean the same thing. And you are supposed to use a sterile/aseptic technique. From what I have read, many visiting nurses do not do that if they are from the doctors' office. But they do it in the hospital. It is always best to do this using a technique that will not infect the patient.
Who might use aseptic technique?
microbiologist,biologist and everyone that need to get a clean sample(prevent adding bacteria to the sample).
What safe practice is part of using aseptic technique?
aseptic technique are all those activity used to prevent the entry of microorganism.
How do you count bacteria using aseptic technique?
dcsddsdsds
What is aseptic transfer?
Transferring bacteria from one place to another and keeping the transfer pure.
Is it possible to contaminate a subculture?
By improperly handling of the bacteria, or by doing any or all steps of the aseptic technique incorrectly.
How is possible to contaminate subculturing?
By improperly handling of the bacteria, or by doing any or all steps of the aseptic technique incorrectly.
Why is learning about the aseptic transfer techniques so important?
Aseptic transfer techniques ensure that when transferring MOs from one place to another, you keep it pure and without other bacteria. If you were to just keep adding more bacteria there is no way you could end up with Pure Culture.
Why is aseptic technique necessary for successful cultivation of pure cultures in the laboratory?
Short answer is Koch's postulates. Long answer you can't ascribe any scientific data you may gather to a bunch of bacteria on a plate or in a culture, as you won't know which of the bacteria gave you the result you observed. So if you are trying to figure out which bacteria causes a disease, inoculating a mix of bacteria won't tell you which one causes the disease. Isolating each one using aseptic technique and then inoculating individually and finding the disease in one animal will tell you which bacteria caused to disease.
What is the difference between aseptic and antiseptic?
The adjectives are partly synonymous, but something that is antiseptic kills bacteria, while something aseptic is "surgically clean or sterile."
A method of aseptic technique is called?
sterilization
When you access a port catheter do you use is sterile technique or aseptic?
The terms sterile and aseptic mean the same thing. And you are supposed to use a sterile/aseptic technique. From what I have read, many visiting nurses do not do that if they are from the doctors' office. But they do it in the hospital. It is always best to do this using a technique that will not infect the patient.
What is the synonyms of aseptic technique?
Infection Control treatment
Who discovered the aseptic technique?
Joseph Jackson Lister
