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Older cultures produce more spores. If the culture is not old enough, the spores will be few, and possibly undetectible.
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What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
What does a positive result for spore stain indicate?
The spore was neutralized before stain removal insued.
What is the counterstain in a spore stain?
the counter stain is safranin 0.5%
Why is it essential to apply heat during endospore staining?
because they cannot be differentiated from inclusions of stored material without a special stain .
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
What does a positive result for spore stain indicate?
The spore was neutralized before stain removal insued.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
How does culture age affect the results of a sproe stain?
Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
What is the counterstain in a spore stain?
the counter stain is safranin 0.5%
What is the gram stain for clostridium perfringens?
Gram positive spore forming rods
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
Why heat the slide when performing spore stain?
Endospores are impermeable to most stains so heat is usually applied to drive the stain into the endospore.
