In the stomach, the pH could be as low as 2. Pepsin is an enzyme that breaks up protein molecules. It needs to have an optimum pH close to that.
In the mouth, amylase occurs. pH in the mouth is often about 7. Optimum pH for amylase needs to be close to that.
Stomach enzymes work best at a very low pH (very acid), but most other enzymes usually work best fairly close to pH 7 or a little higher (slightly alkaline or at any rate not very acid). Notice that this is also the pH of most other parts of the healthy human body.
by increasing the temperature above the optimum temperature(above 45), we can denature an enzyme!! the temperature affects the non-covalent bonds of an enzyme!! at 40
37.5 degree Celsius
They do not work as they effectively change shape and so cannot perform their allocated task
Hydrogen peroxide (H2O2) is a poisonous byproduct of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water (H2O) and oxygen gas (O2).
It means the highest (and best) temperature an enzyme can work until it gets denatured (active site is re-shaped therefore substrate is unable to fit into it anymore). Hope it helped :)
Every enzyme works at its maximum rate at a specific temprature called as optimum temprature for that enzyme. AND all enzymes work at their maximum rate at narrow range of pH, called as optimum pH. A slight increase or decrease in pH causes the retardation in enzyme activity or blocks it completely.
high temperature may denature enzyme activity by passing optimum temperature
by increasing the temperature above the optimum temperature(above 45), we can denature an enzyme!! the temperature affects the non-covalent bonds of an enzyme!! at 40
enzymes best function at optimum temperature for human body optimum temperature is 37 C, raise in optimum temperature affects the function of enzymes and with very high temperature enzyme activity ceases. high temperature also affects proteins resulting in protein structural changes. fever slow down the enzyme activity.
37.5 degree Celsius
The enzyme activity increases as the temperature rises due to the substrates colliding with the enzymes' active sites more frequently at higher temperatures. However, each enzyme has an optimum temperature as high temperatures denature enzymes.
As you increase the temperature at first the activity of an enzymes will also increase, so the reaction will go faster. At a certain temperature, called the optimum temperature, the enzyme will work at its maximum rate. Above the optimum temperature the enzyme activity decreases. This is due to the loss of shape by the enzyme molecules, a process called denaturation. In mammals, most enzyme shave an optimum temperature of about 37 oC. Roughly speaking, the rate of an enzyme reaction doubles for each 10 oC rise in temperature, until the optimum temperature is reached. pH affects enzymes in a similar way. At a low pH many enzymes have a low activity. As the pH increases the enzyme activity increases until the optimum pH at which the enzyme has maximum activity. Above this pH enzyme activity decreases. These effects are also dueto denaturation of the enzyme molecules at extreme pH values. Many enzymes work best at around pH 7.0. An important exception is pepsin, a protein-digesting (protease) enzyme in the stomach. This works at low pH values such as those found in the stomach (around pH 2.0), due to the presence of hydrochloric acid.
The three main factors that affect enjyme activity are:- a)Temperature:Enzymes generally function properly at a narrow rangr of temperature and shows its highest activity t a particular temperature called its optimum temperature. b)pH:-as temperature pH also has a particular value ,in which the enzymes are most active,called the optimum temperature. c)Concentration of substrate;-with the increase in substrate concentration,the velocity of the enzymatic reaction also increases,till a maximum velocitywhich does not rise any further with the rise in substrate concentration.
They do not work as they effectively change shape and so cannot perform their allocated task
Hydrogen peroxide (H2O2) is a poisonous byproduct of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water (H2O) and oxygen gas (O2).
It means the highest (and best) temperature an enzyme can work until it gets denatured (active site is re-shaped therefore substrate is unable to fit into it anymore). Hope it helped :)
55 CThis enzyme works best at a temperature of 50 C and a pH of 12.If the enzyme will be above this temperature, it will denature.