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Answered 2009-04-14 14:50:34

DNA acts as a template for two things: # DNA # mRNA transcripts This is possible because each nitrogenous base in one strand pairs only with one other base in the complementary strand as follows: Adenine (A) always pairs with Thymine (T) Guanine (G) always pairs with Cytosine (C) Note: in RNA, Uracil (U) takes the place of Thymine (T), but still always pairs with Adenine (A). So, if one looks at a single strand of DNA, one can reconstruct the sequence of the complementary strand from the original strand, because each base on one strand will pair with only one base on the other. Consider this single strand of DNA: DNA acts as a template because of one fact: each nitrogenous base in one strand pairs only with one other base in the complementary strand as follows: Adenine always pairs with Thymine (or Uracil in RNA) Guanine always pairs with Cytosine So, if one looks at a single strand of DNA, one can reconstruct the sequence of the complementary strand from the original strand, because each base on one strand will pair with only one base on the other. Consider this single strand of DNA: ATTGCAT Looking at the first base, we know that A always pairs with T, and that T always pairs with A for the second base, and so on. Therefore we can reconstruct the complementary strand is: TAACGTA The mRNA transcript would be: UAACGUA This fact is important in two ways. First, when a cell needs to replicate its DNA, it uses an enzyme, DNA Polymerase, to assemble complementary strands by adding nucleotides with the appropriate matching bases along the strand. The result is two identical, complete DNA molecules. Secondly, when the cell needs to build messenger RNA for protein synthesis, it uses an enzyme called RNA Polymerase to assemble the mRNA transcript by adding RNA nucleotides with the complementary matching bases from the DNA strand. The result is an mRNA transcript which will be translated into a protein whose amino acid sequence will reflect the codon sequence of the original DNA strand.

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What is the template code strands of DNA?

DNA is made of of two complimentary strands, the coding strand and the template strand. When DNA is transcribed (made into messenger RNA which can be converted by ribosomes into proteins) the DNA splits open and free nucleotide bases bind to the template strand. DNA is made of T/C/G/A and RNA is made of U/C/G/A nucleotide bases. G and C bind (they are said to be 'complimentary') A and T bind and in RNA U and A bind (so U replaces T.) The newly formed RNA strand (made on the template stand of DNA) is 'complimentary' to the template but the same as the coding strand of DNA. Hence the template is used to produce RNA which is a copy of the coding strand. Either strand of DNA can act as the template/coding strand. Hope that is a little bit helpful!


Which type of replication takes place in DNA?

DNA replication is called semi-conservative - as the new DNA that is created contains one old strand and one new strand.When DNA replicates, the existing strands separate and act as a template for the new strand to be created.


What molecule makes a mirror copy of DNA?

There is not really such a thing as a mirror image of DNA in nature. DNA polymerase may be the "molecule" that you are thinking of, it is an enzyme that replicates DNA. When the polymerase makes a new strand of DNA, it uses an existing strand of DNA as a template. The new strand of DNA is not in fact a mirror image of the template strand, but it is the closest thing possible. The new strand is called a complementary strand, not a mirror image.Existing DNA (template for polymerase): ATC TGA CCG GAC TAG GGTNew strand (made by polymerase): TAG ACT GGC CTG ATC CCAAlternatively, by mirror image of DNA you may be thinking of RNA, a ribonucleotide that is made by RNA polymerase. The process is similar to that described above, but the new complementary strand is made out of ribonucleic acid rather than deoxyribonucleic acid.


What determines the sequence of the nitrogen bases in DNA strand?

When a new DNA is formed , two strands of old DNA open and act as a template for synthesis of two new strands of DNA .Sequence of bases in new strand of DNA is determined by old strand and it is based on complementarity i.e. A pairs with T and G Pairs with C .


What would be the sequence of DNA that is complementary to a DNA strand with the base sequence of acctgaacgtcgcta?

ACC TGA ACG TCG CTA (Original) TGG ACT TGC AGC GAT (Complentary strand)


Then the two single strands of?

There are two single strands which are found in the DNA molecule. Each of strand will act as the template for the next one in the double helix molecule.


What strand of mrna would be produced from the strand of DNA act gac?

UGA CUG


Which event marks the beginning of DNA replication?

a) The Hydrogen bonds between the complementary bases break, allowing the DNA helicase to unzip b) Each single DNA strand act as template to build a complementary strand. c) finally any errors are repaired, resulting into two identical DNA molecule, one for each daughter cell.


If a DNA strand sequence is tacgccagtggttcgatc what will be the complimentary strand?

gat cga acc act ggc gta.


When forming new DNA molecules each strand of DNA your act as a?

When your DNA replicates itself, the needed section of the double helix "unzips" into two strands. Each of those strands is called a template. Free nucleotides assemble onto the templates using base pairing with the enzyme DNA polymerase and create a new double helix.


A segment of DNA shows bases in this order agt cac gca complete the corresponding DNA strand tca gtg?

It is wrong. The corresponding DNA strand is: 5' tgc gtg act 3' because you have to do the complementary and then revert it.


What strand of mRNA would be produced from the strand of DNA shown below?

Answer this question… ACT GACA. ACT GAC B. TGA CTG C. AGU CAG D. UGA CUG


What are the functions of the following enzymes helicaseDNA polymerase RNA polymerase?

helicases are necessary for unwinding double stranded DNA. in order to replicate its DNA, the cell must first unwind the two strands. once the the two strands have been separated enzymes such as DNA polymerase and RNA polymerase can act on the single stranded DNA. DNA polymerase creates a strand of DNA from a template strand of DNA. it is used to create a new copy of the DNA. In order to begin DNA synthesis, a primer is necessary. The primer is a short oligo nucleotide fragment that hybridizes to the template strand of DNA (the strand which is to be copied). Once the primer is hybridized, the DNA polymerase can extend the primer and add nucelotides in a Watson-crick base pairing fashion. DNA polymerases always extend the primer in the 5' to 3' direction. RNA polymerases are used to transcribe genes from DNA. Transcribtion is the process of making an RNA copy of information encoded in DNA. Once a gene is transcribed and a messenger RNA (mRNA) is produced, that mRNA can leave the nucleus to be translated into a protein by the ribosome. RNA polymerases work in a somewhat similar fashion to DNA polymerases, save for the fact that they produce RNA from DNA, instead of producing a new copy of the DNA.


Which of the following is true about DNA?

Both of the original DNA strands act as templates during replication


What is 5' and 3' in DNA?

5' and 3' are referring to the different ends of a DNA strand. The 3' ("three prime") end has a hydroxyl group (OH) and the 5' ("five prime") end has a phosphate group. Every DNA strand has both a 3' and a 5' end. This means that the two ends of DNA can act differently in relation to things like enzymes (such as DNA Polymerase).


What are the different ways restriction enzymes can act on DNA?

Restriction enzymes (REN's) cut or cleve DNA. This cutting process is sequence-specific. Which means, the enzyme does not randomly fragment the DNA (or template strand as it is generally called in many experimental protocols). Instead, the enzyme scans the length of the template until it reaches a specific sequence of 4 - 8 nucleotides. Once this sequence (the restriction site) is reached, the enzyme produces a cut in the template and generates either blunt ends (no overhangs) or sticky ends (having both 3' and 5' overhangs) The primary application of restriction enzymes is is genetic engineering where we want to insert cDNA sequences into plasmids or other vectors.


What does helicase do in replication?

Helicase breaks the hydrogen bonds holding the complementary bases of DNA together (A with T, C with G). The separation of the two single strands of DNA creates a โ€˜Yโ€™ shape called a replication โ€˜forkโ€™. The two separated strands will act as templates for making the new strands of DNA. One oriented in the 3โ€™ to 5โ€™ direction, is the leading strand. The other oriented in the 5โ€™ to 3โ€™ direction is the lagging strand.


What does the DNA helicase do to the DNA?

Helicase breaks the hydrogen bonds holding the complementary bases of DNA together (A with T, C with G). The separation of the two single strands of DNA creates a โ€˜Yโ€™ shape called a replication โ€˜forkโ€™. The two separated strands will act as templates for making the new strands of DNA. One oriented in the 3โ€™ to 5โ€™ direction, is the leading strand. The other oriented in the 5โ€™ to 3โ€™ direction is the lagging strand.


What is the process of copying an RNA message from DNA?

Transcription, I remember this because transcribing is the act of copying an original.


Why is RNA necessary to act as massenger?

it is because DNA can be damaged out side the cell nucleus, which leads to cancer so it replicates it self to stop damage to the original DNA


How is information continued within a gene?

Cell replication. Cells do not live forever, and in light of this, they must pass their genetic information on to new cells, and be able to replicate the DNA to be passed on to offspring. It is also required that fragments of DNA (genes) have to be copied to code for particular bodily function. It is essential that the replication of it is EXACT. In order for replication to occur, the following must be available * The actual DNA to act as an exact template * A pool of relevant and freely available nucleotides * A supply of the relevant enzymes to stimulate reaction * ATP to provide energy for these reactions When replicating, the double helix structure uncoils so that each strand of DNA can be exposed. When they uncoil, the nucleotides are exposed so that the freely available nucleotides can pair up with them. When all nucleotides are paired up with their new partners, they re-coil into the double helix. As there are two strands of DNA involved in replication, the first double helix produces 2 copies of itself via each strand. It is said that the replicated DNA is semi-conservative, because it possesses 50% of the original genetic material from its parent. These 2 new copies have the exact DNA that was in the previous one. This template technique allows genetic information to be passed from cell to cell and from parents to offspring.


What is a topoisomerase?

Topoisomerase: are isomerase enzymes that act on the topology of DNAHelicase untwists the double helix and separates the template DNA strands at the replication fork. This untwisting causes tighter twisting ahead of the replication fork, and topoisomerase helps relieve this strain


Why does a DNA molecule differ from the rna molecule?

Generally the DNA molecule is double stranded to RNA's single strand. The RNA molecule uses uracil as a base while the DNA molecule uses thymine. RNA has catabolic properties that allow it to act in things such a ribosomes and tRNA. DNA is just a carrier of the genetic information.


What does it mean when it is said that DNA is the template?

When DNA replicates, it is "unzipped", or split in half. Each half will be left with only one half of a base pair (A&T, C&G). To make new DNA, the single helix will act as a template to build the complementary single helix, which will in turn create a new double helix. Since each element of a base pair can only pair with its other half, the helix is a map, or template, for the new helix. The same concept applies when talking about transcription, or the formation of RNA. However, RNA replaces thymine (T) with uracil (U).


What job do the enzymes helicase play?

Helicase breaks the hydrogen bonds holding the complementary bases of DNA together (A with T, C with G). The separation of the two single strands of DNA creates a โ€˜Yโ€™ shape called a replication โ€˜forkโ€™. The two separated strands will act as templates for making the new strands of DNA. One oriented in the 3โ€™ to 5โ€™ direction, is the leading strand. The other oriented in the 5โ€™ to 3โ€™ direction is the lagging strand.


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