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cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
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∙ 12y ago
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∙ 8y agoDNA can be cut into pieces by endonucleases, some of which are called "restriction enzymes".
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∙ 9y agoRestriction enzymes are typically used to cut DNA into fragments. There are many different restriction enzymes including EcoRI that are used.
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∙ 13y agoDNA are cut into amsll fragments with the use restriction enzymes.Since this enzymes cuts the DNA into fragments it is also called molecular scissors.
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∙ 13y agoEnzymes called DNAses fragment DNA. DNA may also be denatured by high temepratures, acidity, etc
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∙ 11y agoA restriction enzyme. Used by bacteria to cut up viral DNA but co-opted for recombination work these days.
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∙ 13y agoRestriction enzymes chop up the DNA
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∙ 10y agoRestriction enzymes.
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Does a restriction enzyme generate the same size fragments in genomic DNA of different species?
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Which restriction enzymes cuts the smallest pieces of DNA?
In a practical application, we need a Book; it will also say which restriction enzymes leave the longest fragments.
How do you use the DNA fragments on bakugan dimensions?
You have to collect 20 DNA fragments to get a free bakugan
What stand of DNA replicated in okasaki fragments?
The lagging strand of DNA contains okazaki fragments, which are later joined by DNA ligase
When a restriction enzyme cuts a dna molecule the cuts are staggered so that the dna fragments have single stranded ends why is this is important in recombinant dna work?
It is important because the fragments will bond to other fragments with complementary single-stranded ends.
Does a restriction enzyme generate the same size fragments in genomic DNA of different species?
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
How do you get DNA fragments in Bakugan dimensions?
You get DNA fragments by entering Bakugan codes.
Which restriction enzymes cuts the smallest pieces of DNA?
In a practical application, we need a Book; it will also say which restriction enzymes leave the longest fragments.
What do you do with DNA fragments in bakugan dimensions?
When You collect 20 DNA fragments you get a free bakugan
What are DNA fragments that have attached RNA fragments called?
Okazaki fragments.
What enzyme binds fragments of DNA on the lagging strand?
The enzyme that is used to bind DNA fragments together is DNA ligase. Using DNA ligase to join DNA fragments is the last step in the production of a recombinant DNA plasmid.
How do you use the DNA fragments on bakugan dimensions?
You have to collect 20 DNA fragments to get a free bakugan
Who has exactly the same DNA restriction fragments?
two identical DNA fragments will have identical restriction fragments. Also, genetically identical twins will have identical restriction fragments
What stand of DNA replicated in okasaki fragments?
The lagging strand of DNA contains okazaki fragments, which are later joined by DNA ligase
