What is a okazaki fragment?

Answer 1

Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction)

never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction.

The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'.

Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase.

Here I attache a link to a flash video that will make u understand better!

http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf

Answer 2

Okazaki fragments was discovered by Reiji Okazaki and collegues.During replication of DNA molecules lagging strand is synthesised on 5'->3'strandof DNA in short segments of 1000 to 2000 NT in 5'->3' direction.These segments are called Okazaki fragment. Synthesis of each Okazaki fragment begins with RNA primer synthesised by primase and carried out by DNA polymerase |.

Answer 3

okazaki fragments are new pieces of Dna On the lagging strand

Answer 4

an okazaki fragment is 1000-2000 of neucleotides long in E-coli and 100-200 nucleotides long in eukaryotic cells..

Answer 5

Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication

Answer 6

5mg to 100mg plasmids prepration CGMD grade DNA