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Q: Is Bacterial Artificial Chromosome DNA low copy or high copy?
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Why pBR322 plasmid have high copy number?

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HIgh copy number means that the bacteria will replicate many copies of the plasmid. That means that you suppose to get a high yield of plasmid at the end.


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Is plasmid that can replicate in Ecoli and Pseudomonas a high copy number plasmid?

Many Pseudomonas plasmids are transmissible to E. coli, but the copy number is plasmid-dependent. Some plasmids are of low-copy number whereas some are high-copy number, and we should also consider copy-number-incompatibility. It could not be said that plasmids which can replicate in E.coli and Pseudomonas are of high copy number.


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