why RT was shifting & how to RT calculation in HPLC
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
HPLC
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
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When you increase the flowrate of the carrier gas, the retention times decrease. Just like when you increase the temperature of the column. Both of these conditions are sometimes necessary for substances that would otherwise have very long retention times.
concentration of buffer effects in buffer strength
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
the difference between retention time and retention factor is that the retention time is a quatitative value where`s the retention factor is a qualitative on.
Retention time decrease with an increase in pH.