it depends greatly on the kind of chromatography.
This answer will deal with the two ways i use acetic acid in silica gel chromatography.
1) most often i use acetic acid when i need to separate/elute carboxylic acids from each other or from other compounds on silica gel. carboxylic acids donate protons pretty readily on silica, and thus become ionized. when ionized they do not move on silica gel. By adding between 0.1% and 10% acetic acid to my solvent mix, i can keep the carboxylic acid protonated so that it will elute off of silica gel like any other organic compound. the acetic acid can then be evaporated away just like any other organic solvent under reduced pressure.
2) for particularly polar organic comppounds, i sometimes use acetic acid as the polar component of my solvent mix, but this is rare-- I've only used it this way 5-7 times in the last 8 years.
in both cases above the exact amount of acetic acid added to my solvent mix (also called the mobile phase) has to be determined experimentally by trial and error.
when i have a relatively non-polar carboxylic acid to isolate, i may start with 85% hexane, 14% Ethyl acetate, 1% acetic acid.
if i have a more polar carboxylic acid to isolate, i might start with 95% dichloromethane, 4% methanol and 15 acetic acid.
I'll try a TLC at that mix, and then visualize the migration of the carboxylic acid by UV if it is UV active, or by staining with bromocresol green if it is not (remember to completely and thoroughly dry the TLC plate before staining -- to evaporate all the acetic acid since it will stain positive with bromocresol green too-- and remember to heat the stained TLC plate (about 20-200 seconds at 200C should work).
trifluoroacetic acid (TFA) also often added at a rate of about 0.1% to mixtures of acetonitrile and water for use in HPLC, BUT when the HPLC is coupled to a mass spectrometer (LCMS) use of trifluoroacetic acid can decrease analyte signal, so 0.1% acetic acid is often substituted.
First, there is no single substance that is identified as "acid." An acid can be one of many different substances, with a variety of different properties.
Although acids can be used as solvents they are more often solutes, as they are more often found dissolved in water than in their pure forms.
Acetic acid and water can mix together into solution in any ratio you want, so this answer's going to look a little strange.
Acetic acid will dissolve into water in any ratio from 1 percent acetic plus 99 percent water, to 50/50 acetic and water. When the concentration of acetic acid exceeds 50 percent, the acid no longer dissolves into the water--because the product with the highest concentration is considered the solvent, at 51 percent acetic acid you should think of the solubility of cold water in acetic acid. And at that point, you can have any ratio from 51 percent acetic plus 49 percent water, to 99 percent acetic plus 1 percent water.
acetic acid is a weak acid it is used as agent in a chemical reactions.some times used as a solvent to dissolve the compounds
Yes, acetic acid is water soluble in all proportions.
Vinegar is acetic acid (4%) dissolved in water. By observation - it is soluble. Water free (anhydrous) acetic acid (AKA ethanoic acid) is a solid crystal known as glacial acetic acid.
Acetic acid is very polar and water is a polar solvent.
Acetic acid is completely miscible in ether. That means acetic acid can mix in all proportions with ether. They make a homogeneous mixture when dissolved.
yes it is quite soluble
Acetic Acid is insoluble in dil. HCl
aqueous acid solution it is solute or solvent
Water is the solvent and acetic acid is the solute.
The aqueous solution of HCl is the Muriatic acid so HCl gas is solute and water is solvent.
Acid or alkali
It could be either or neither.
aqueous acid solution it is solute or solvent
solvent acid solut frog
Water is the solvent and acetic acid is the solute.
Phosphoric acid and chromic acid
The aqueous solution of HCl is the Muriatic acid so HCl gas is solute and water is solvent.
The aqueous solution of HCl is the Muriatic acid so HCl gas is solute and water is solvent.
Pivalic Acid
Acid or alkali
It could be either or neither.
No, ammonia is a base and can be used as a solvent.
Maybe, Methanol
Lactosic acid