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Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.

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9y ago
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9y ago

Eosin is an acidic stain with a negatively charged chromosphere. Methylene blue is a basic stain with a positively charged chromosphere. Since bacterial cells are usually negatively charged, the cells will attract the methylene blue and repel the eosin making the cells blue and the background red.

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eosin is acidic and meth blue is basic in this case eosin acts as a neg stain. the cytoplasm will be red and the cell will be blue.

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For differentiating different types of cells in an organ or organism

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Q: Staining preparation with eosin and methylene blue?
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Related questions

What is the standard stain for biological tissues?

Eosin and methylene blue are used for staining biolgical tissues.


What is the function on bile in Eosin Methylene Blue agar?

To make the Eosin Methylene BLue Agar look cool and hip.


What kind of stain use in histology?

It depends on what tissue you're looking at, what you want to stain, how the tissue has been stored... Besides very specific staining, there are different types of staining. For example, immunohistochemistry, which uses antibodies to stick coloured stains to cell surface receptors. Or, chemical staining - the most common is H&E staining (haemotoxylin & eosin), so if you're just having fun in a lab and want to see general structures of cells, use this one.


What is the composition of leishman stain?

Polychromed methylene blue Eosin Azure Leishman powder Alcohol free absolute alcohol


What is Eosin Dye?

It is a dye found in Eosin-methylene blue (EMB) agar, which permits the differentiation between enteric lactose fermenters and nonfermenters. The dye methylene blue inhibits the growth of gram positive bacteria. While the eosin acts as a pH indicator, measuring the levels of acid production due to the fermentation of lactose.Eosin is a stain that is used for staining the cells to observe the structurea nd number of cells. It is an acidophilic stain.


What function of emb agar?

function of eosin methylene blue agar (EMB)


What color is simple staining on a bacillus and coccus using methylene blue stain?

Methylene blue stains everything blue.


What is thee purpose of adding methylene blue to the specimen?

Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.


When staining ecoli with methylene blue what would you see?

blue bacilli


Eosin methylene blue agar?

yes , Escherichia coli and Enterobacteraerogenes can grow on it.


Chemical used to make a specimen visible?

A general term for a chemical that makes a specimen visible is a stain. There are many types of stains available, depending upon the structure you want to visualize and the type of microscope you want to use, e.g. fluorescent stains like DAPI for fluorescence microscopy, or hematoxylin and eosin (H and E) staining for brightfield microscopy.Immersion oil


What is embbed?

Eosin Methylene Blue Agar (EMB) was developed by Holt-Harris and Teague.1 This formula contains lactose and sucrose with two indicator dyes, Eosin Y and Methylene Blue. The use of Eosin Y and Methylene Blue as indicators produced sharp and distinct differentiation between colonies of lactose fermenting and nonfermenting organisms. Sucrose is included to detect coliforms that ferment sucrose more readily than lactose. EMB Agar is selective due to the presence of an inhibitor and differential based on the ability of some organisms to ferment carbohydrates with the absorption of an Eosin Y and Methylene Blue complex.